Course: Principles of Biochemistry I & Biochemistry I Class: BS- Bioinformatics & BS-Biosciences Lab Instructor: Ms. Sadia Jabeen Lab Protocol #
Free Glucose Carbohydrate Disaccharide
The limiting reagent it CuCl2 because it is the one that will run out first compared to the .009 moles of Na2Cl3‚ which it the excess reagent because it is a higher amount of moles compared to the .007 moles of the CuCl2. The amount of excess reagent in grams that should remain in solution if the theoretical yield of CuCO3 is produced is: 1 mole of CuCl2 (63.55) +(2*35.45) =134
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As a teenager‚ Lucas’ mother was a prostitute and caused him to hate women by forcing him to be a bystander while she performed sexual acts with various men. This was ongoing occurrence for years‚ but as Lucas got older he would often go home late or not go home at all in hopes of missing this common practice of his mother’s. There would be consequences once Lucas did return home for missing his mother’s acts such as beatings. His first crime
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Determination of presence of protein in a variety of food substances by the use of Biuret Reagent Biol. 1040 Section 1005 Introduction The human body consumes carbohydrates‚ lipids‚ and proteins each day by eating a variety of foods. These foods are required for energy and human growth. Proteins are a diverse group of macromolecules with many different functions (Mbuthia‚ 2012). Many are structural components of bone‚ muscle‚ hair‚ tissue‚ etc. Others are enzymes that speed up cellular
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the ninth tube was labelled ‘Negative control. all the sample tubes 1b‚ 2b‚ 3b and positive control b‚ 0.5 ml of reagent 1 and 50 µl of reagent 2 and 0.5ml of saline was added ‚and To the sample tubes 1a‚ 2a and 3a and positive control a‚ 1.0 ml of 0.9% saline and 50 µl of reagent 2 was added to them and To the negative control tube‚ 600 µl 0.9% saline‚ 0.5 ml reagent 1 and 50 µl reagent 2 was added and all 9 tubes were placed in 37oC water bath for 15 minutes to allow the reaction to finish‚ the
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Biuret Reagent 15. Iodine 16. Benedicts Reagent Methods In this lab there are I complete two different tests. The procedure was relatively the same for each test. I would first add 3ml of a given substance to a test tube‚ followed by 5 drops of either Biuret Reagent or Iodine. Once the agent is added‚ I swirled the two substances and then observed the results. The first was to test proteins‚ and the second‚ was to test starches. In the first test‚ Proteins‚ I used biuret reagent to determine
Free Glucose Carbohydrate Enzyme
To determine if the reaction had gone to completion‚ a Starch/Iodine test was conducted. A change in the strip to a deep blue color would signal an excess of sodium hypochlorite reagent present. An excess is the reagent symbolizes the end of the reaction because there is no longer any reactant left to consume reagent. After the reaction was deemed completed‚ the product was isolated and purified by ether extraction‚ aqueous extraction and evaporation. To determine how sodium hypochlorite acts as
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To determine protein concentration in apple juice. Procedure Part1 7 test tubes were prepared and labeled. The appropriate amount of BSA and distilled water were pipetted into the respective test tubes according Table 3.1. 500µl of Branford Reagent were added into all the test tubes. The test tubes were all vortexed and mixed.
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The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution [1]. There are several reagents that can be used to determine the concentration of protein in solution which are Bradford reagent‚ Biuret reagent‚ Lowry protein assay and many more. In this experiment‚ the Bradford Reagent is used to measure the protein concentration. Marion M. Bradford had developed the Bradford protein assay. The Bradford assay is a colorimetric assay based
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Synthesis and Structure of Alcohols Alcohols can be considered organic analogues of water. H O H R O H Alcohols are usually classified as primary‚ secondary and tertiary. H R H OH H primary R R OH R R OH OH R secondary tertiary phenol Alcohols with the hydroxyl bound directly to an aromatic (benzene) ring are called phenols. Nomenclature of Alcohols (Normally any compound’s name which ends in –ol is an alcohol of some sort) IUPAC rules that:
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