Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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they returned to England. They worked as student-teachers at the Pensionnant Heger‚ run by Mme Claire Zoe Heger and her husband Constantin. She developed a deep attachment towards Constantin and she used him as inspiration to create the character of M. Paul Emanuel. “A dark little man he certainly was; pungent and austere. Even to me he seemed a hard apparition‚ with his close-shorn‚ black head‚ his broad‚ sallow brow‚ his thin cheek‚ his wide and quivering nostril‚ his thorough glance and his hurried
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Big-M method The Big-M method of handling instances with artificial variables is the “common sense approach”. Essentially‚ the notion is to make the artificial variables‚ through their coefficients in the objective function‚ so costly or unprofitable that any feasible solution to the real problem would be preferred....unless the original instance possessed no feasible solutions at all. But this means that we need to assign‚ in the objective function‚ coefficients to the artificial variables that
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How many of you like M&M’s? I’m sure a lot of you do‚ but do you know how they were introduced to us? Well‚ let me tell you. Forrest Mars came up with the idea of creating the now famous M&M’s. Mars saw soldiers eating pieces of chocolate covered with a hard sugary coating. The coating preventing the candy from melting in the hot sun. In 1940‚ he made his first‚ independent move into the candy business in the United States. Somewhere in his travels‚ Mars had come across a candy that was essentially
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making considerable investments to take advantage of the new business possibilities offered by wireless technologies - encouraged by optimistic‚ yet contradictory forecast on the future volume of m-commerce. This paper highlights the importance of setting out from a consumer perspective when developing m-commerce strategies‚ proposing an analytical framework that can be used to assess whether‚ and in what ways‚ specific mobile services are likely to offer value for wireless Internet users. The paper
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M is for Me‚ I’m a kid that is 12 years old and likes soccer. My life is okay‚but there is more downs than ups in my life. I have more downs because I’m not really good at other things than soccer and I’m disliked a lot‚but I don’t know why I’m nice‚ cool‚ and people can talk to me about anything. I’m a good person to be with‚ you can talk to me about anything‚ we play sports or anything you want. I’m very intelligent so ask me for questions about homework and I’ll will help you‚ I could help anyone
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treatment by biological activated sludge system it was found out that the k value was 4.46 d-1‚ based on sBOD. The authors also noted that the maximum substrate utilization rate value for domestic wastewater and k value for cheese processing is 5 g sCOD/(g VSS∙h) and 9.3 d-1‚ respectively‚ showing that k value was lower for dairy wastewater due to the difference in wastewater composition. Therefore‚ in the present study‚ k value (0.34 h-1 or 8.16 d-1) neatly fit with that obtained in the cited study
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organize their time in one of two ways: monochronic (M-time) or polychromic (P-time). Both of these classifications represent very distinct approaches to time utilization and perception of time‚ one being monochronic‚ which views time as segmented and something that should be rationed. Whereas the other‚ polychronic‚ sees time as something that should be flexible‚ so the more important things in life can be given more attention. Considering M-time cultures are extremely punctual and use time judiciously
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FASHION MARKETING & COMMUNICATION General Overview General Overview H&M Hennes & Mauritz AB is a Swedish global retail-clothing company‚ known for its fast-fashion. 2‚500 stores are spread across 44 markets. Germany is H&M’s largest market‚ followed by the US‚ France and the UK. H&M has more than 20 country offices that are responsible for the various departments in each sales country. local H&M production offices managing contacts with approximately 700 independent suppliers
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Mergers & Acquisitions (M&A) in Indian Telecom Industry Submitted by: Akshat Mittal NSIT‚ Delhi 1. Introduction: Indian has achieved an overall teledensity of 67% and rural teledensity of 31%. Indian telecom industry is fast becoming an attractive destination for foreign investors and is experiencing consolidation of operations. It has witnessed several multi-billion dollar M&A deals ever since the telecom industry was opened to the private players in 1995. In the Indian
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