and the survival of the bacteria. Control: Nutrient agar plate with streaks of Serratia Marcescens without exposure to UV light. Results: Figure 1: Represents the nutrient agar plate used as the control that was not exposed to the UV light. Each quadrant represents a 10 sec exposure to the bacteria increasing to 40 secs. Serratia Marcescens bacteria streaked Figure 2: Represents the nutrient agar plate used as the second control with the bacteria that
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code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the cells‚ bacteria treated with the plasmid were grown on two separate agar plates containing LB nutrient broth and ampicillin‚ and another containing LB nutrient broth‚ ampicillin
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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This study was conducted to isolate and culture Dictyostelids from the soil to observe this unique life cycle. Soil sample was collected and diluted to volume. A measure of 0.6 mL of the soil suspension were put into a 1/3 stength of Hay Infusion Agar. Then‚ 0.4 mL of 24-hour old E. coli was added to serve as nutrient source of the Dictyostelids. The plates were incubated at 24°-25°C for 24 hours and were observed periodically the second day. Using a compound light microscope‚ the development of
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Enumeration of Bacterial Contamination in Hamburger Meat from Unknown Sources C March 6‚ 2012 The importance of bacterial enumeration has become even more apparent in recent years due to the increasing numbers of harmful bacteria found in meat products. This process is the key to understanding the populations of microorganisms that contaminate the food supply. Much of the bacteria in meat has been shown to be resistant to multiple drugs; so disease-causing microbes
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Effects of Crossing Over in Sordaria fimicola Abstract Sexual reproduction in organisms is a cause for genetic variation. This can be seen through the process of meiosis in Sordaria fimicola because of the effects of crossing over and independent assortment that occur in meiosis I. Before performing this experiment we hypothesized that we would be able to see that crossing over did indeed occur in the S. fimicola. We tested our hypothesis by growing our own culture and observing it under a
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THE EFFICACY OF TAKIP-KOHOL (Centella asiatica) LEAVES EXTRACT AS ANTIBACTERIAL AGENT AGAINST Escherichia coli‚ Salmonella enteritidis AND Staphylococcus aureus CLUSTER 1 LIFE SCIENCE TEAM CATEGORY 1|Page28 ABSTRACT THE EFFICACY OF TAKIP-KOHOL AGAINST (Centella Escherichia asiatica) coli‚ LEAVES Salmonella EXTRACT enteritidis AS AND ANTIBACTERIAL Staphylococcus aureus AGENT This research study aims to discover the efficacy of Takip-kohol leaves extract as antibacterial agent against Escherichia
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Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three others in order to identify the changes
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of E. Coli‚ Agar‚ LB and Ampicillin and we expect the transformation efficiency to be zero because of the absence of a plasmid. The experimental group is the same as the control group except the plasmid is included. Thus‚ the only difference between the control and the experimental group is the presence of plasmid. The transformation efficiency was calculated in order to determine the impact the plasmid has on transformation by dividing the total number of colonies growing on the agar plate by the
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Introduction To date‚ the bacterial resistance to antibiotics is one of the most important global health threat introduced by the World Health Organization (WHO) and most annual mortality due to hospital infections occurs because of this challenge (1). According to the US Centers for Disease Control and Prevention (CDC) report‚ carbapenem-resistant Enterobacteriaceae (CRE) are one of the three main antibiotic resistance threats. (2). Enterobacteriaceae are Gram-negative bacilli which cause a wide
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