EXPERIMENT 8 THIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES Additional Resources http://orgchem.colorado.edu/hndbksupport/TLC/TLC.html http://coffeefaq.com/caffaq.html Materials Needed TLC equipment: 1 5-cm x 8-cm TLC plate‚ 2 capillary micropipettes‚ TLC tank and lid‚ ruler‚ UV lamp Extraction Equipment: spatula‚ 2 small test tubes‚ Pasteur pipet and bulb Chemicals: caffeine‚ ethanol‚ dichloromethane‚ TLC solvent (5% acetic acid in ethyl acetate)
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What is Chemical and Process Engineering? The branch of Engineering which is concerned with processes in which materials undergo a required change in composition‚ energy content or physical state at a reasonable cost and in the safest possible manner. History of Chemical Engineering For all intents and purposes the chemical engineering profession began in 1888. While‚ the term "chemical engineer" had been floating around technical circles throughout the 1880’s‚ there was no formal education
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neutralization. HCl(aq) + Fe(s) C5H10 + O2(g) Pb(NO3)2(aq) + FeCl3(aq) HBr(aq) + MgO(s) KI(aq) + Pb(NO3)2(aq) Predict the products for the following chemical reaction. Write balanced molecular‚ complete ionic‚ and net ionic equations. Na2CO3(aq) + HCl(aq) ? Chapter 8 2.00 g of ammonia (NH3) are combined with 5.00 g of molecular oxygen to produce nitrogen monoxide and water vapor. Find the limiting reactant and theoretical yield for this reaction. If 2.50 g of NO are collected‚ what
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Hydrogel dressing in comparison with hydrocolloidIntroductionWound repair may be divided into three overlapping phases‚ namely the inflammation granulation‚ and the matrix formation and re-modelling phases. In the inflammation phase‚ macrophages participate in the cleansing of the wound and are also responsible for initiating angiogenesis and the appearance of fibroblasts through the action of the cytokines they release. (Panchgnula and Thomas 2000 131-50) In the second phase of wound healing‚ granulation
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Last Name ____________________First Name _______________________ Ignore: 5‚ 7‚ 19‚ 30 1. | How many grams of CaCl2 (molar mass = 111.0 g/mol) are needed to prepare 4.44 L of 0.500 M CaCl2 solution? | | A) 369 g B) 271 g C) 258 g D) 296 g E) 246 g | 2. | An aqueous solution of ammonium sulfate is allowed to react with an aqueous solution of lead(II) nitrate.The complete ionic equation contains which of the following species (when balanced in standard form)? | | A)
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D: Introduction: In this experiment calcium carbonate will be put into a flask and mixed with hydrochloric acid to produce calcium chloride‚ water and carbon dioxide. The formula for this reaction is: CaCO3(s) + 2HCl(aq) → CaCl2(aq) + CO2(g) + H2O(l) Purpose: The purpose of this experiment is to determine how the surface area of CaCO3(s) affects the rate of reaction by measuring the volume of CO2(g) produced with time. Background: When solid reactants
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Module One: Grade Nine Topics Physical Change: This is a usually reversible change in which the form of matter is altered‚ but is not chemically changed to another substance. Melting an ice cube is an example of a physical change. The ice cube changes states: from a solid to a liquid (water). You can physically see this change. No new substance is formed: the matter in the liquid is the same as it was in the ice cube. It is reversible: you can freeze the liquid to turn it back to ice.
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Gel filtration chromatography on Sephadex G-50 The crude broth obtained after fermentation was subjected to ammonium sulphate precipitation at 70% (w/v). The pellet so obtained was resuspended in cold saline (2 ml) and dialysed. The dialysed enzyme was loaded onto a column of Sephadex G-50 (120 cm × 1.0 cm) equilibrated with 10 mM Tris-HCl buffer‚ pH 8. The column was eluted at a flow rate of 1 ml / 6 min. The elution profile of gel filtration chromatography is shown in the (Fig: 1). The fractions
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was determined by red color of its flame. Unknown 1 was strontium chloride and unknown 2 was calcium chloride. The unknowns were found by comparing their flame colors to the known substances. Formula | Metal | Color Observed | Duration(sec) | CaCl2 | Calcium Chloride | Green | N/A | CuCl | Copper Chloride | Orange | N/A | FeCl3 | Iron Chloride | Sparkle | 6 sec | LiCl | Lithium Chloride | Pink | N/A | KCl | Potassium Chloride | Purple | N/A | NaCl | Sodium Chloride | Orange | 30 sec
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Thirty test tubes One test tube rack Two test tube holders Two spatulas Three 250 mL beakers One stirring rod One medicine dropper Alcohol lamp or Busen burner Water bath One looped platinum or nichrome wire Label REAGENTS 0.5 M CaCl2 0.5 M BaCl2 M NaOH 0.1 M AgNO3 0.5 M KCl 0.5 M KBr 0.5 M KI 2 M NH4OH M KCN % H2O2 2 M H2SO4 MnO2 Saturated FeSO4 0.1 M NaNO3 CH3COOH 0.5 M Na2SO3 0.1 M KSCN 2 N KOH 0.5 M K4[Fe(CN)6] 0.5 M FeSO4 0.5 M FeCl3 0.5 M Al2(SO4)3
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