investigate the effect of temperature on the enzyme catalase. The original research question was exploring the effect temperature would have on a yeast catalase reacting with hydrogen peroxide (H2O2). To address the latter question a series of experiments were conducted. The various temperatures experimented with were as follows: 22 degrees Celsius (room temperature)‚ 0 degrees Celsius (freezing)‚ 100 degrees Celsius (boiling)‚ and 37 degrees Celsius. Along with variations in temperature‚ the experiment involved
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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collected at the beginning of experiment was found to have a pH value of 7(neutral) ‚ as expected since the normal pH of saliva is 6 to 7 (Humphrey and Williamson‚ 2001). When testing the enzymatic action of saliva‚ we observed at the end of the experiment‚ that a yellow-red precipitate formed which indicated that sugars were present. The reason that sugars were found and not starch‚ is because saliva contains an enzyme known as salivary amylase which catalyses the breakdown of starch to produce sugars
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Effect of Temperature on Enzymes ~Abstract~ In this experiment the effect of different types of temperatures on enzyme activity was examined. The temperature baths that were used to test the difference in enzyme activity on fresh liver were; 4 °C‚ room temperature which was 22°C‚ body temperature which is 37°C‚ and 77°C. The total time of each trial was 2 and a half minute‚ 1 minute for the H2O2 to acclimatize to the temperature‚ 1 and a half minutes for the reaction to occur. Catalase causes Hydrogen
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selected. Select the BAR CHART tab and turn on Show numerical values. 1. What is the initial temperature of each beaker? Beaker A 95 Beaker B 5 2. Click Play ([pic]) and observe. A. What happens to the temperature of Beaker A over time? Beaker a goes down over time. B. What happens to the temperature of Beaker B over time? Beaker b goes up. 3. Why do you think the temperatures of Beaker A and Beaker B changed as they did? Because of conduction. |Activity A:
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phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate; these factors include temperature‚ pH levels‚ chemical substances and the concentration of both
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Investigating the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed
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Goldfish and Water Temperature Goldfish and Water Temperature The subject of my observation is a goldfish that is kept in a 2 gallon fishbowl positioned on a window side table. I have noticed that the goldfish is more active in the earlier morning and later evening than during the afternoon hours. Because the table is near a window that faces northeast‚ the fishbowl receives direct sunlight during the time between eleven o’clock a.m. and four o’clock p.m. The goldfish activity decreases around
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AIR POLLUTION IN THE PHILIPPINES Guides and Tips Home / Blog / Air Pollution in the Philippines Air pollution is a common and frequent problem nowadays. When you are on your way to work‚ you pass by your neighbors’ backyard where he is currently burning their garbage from yesterday‚ then you come across a smoke-belching public utility vehicle‚ then a guy beside you lighted a cigarette and started suffocating you with second-hand smoke. You cannot always depend on your handkerchief on covering
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Measure and record initial temperature for both hot and cold for 2-3 minutes a. b. quickly lift lid and add hot water to the cold recover‚ stir and note mixing time 5. Record temperature every 30 seconds for 20 minutes or until temperature reaches its max. 6. Empty and dry all equipment 7. Place 50.0 ml NaOH in Styrofoam cup 8. Place 50.0 ml assigned acid (phosphorc acid) in beaker 9. Repeat steps 4-6 10. 11. Prepare computer apparatus Set the vertical axis for temperature and the horizontal for time
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