Enzymatic Assay of TYROSINASE (EC 1.14.18.1) PRINCIPLE: L-Tyrosine + O2 L-DOPA Tyrosinase Tyrosinase > L-DOPA > L-DOPA-quinone + H20 Abbreviation used: L-DOPA = L-3‚4-Dihydroxyphenylalanine CONDITIONS: METHOD: REAGENTS: A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine
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The resistance of a wire Planning:- Introduction: To investigate the resistance of a wire at different lengths to see whether or not a longer wire has a larger resistance than a shorter wire. Apparatus: Wire Voltmeter Ammeter Battery 2 crocodile clips Metre rule Variable resistor Diagram: Variables: Length Thickness Resistance Material The material and diameter of the wire needs to be kept constant to keep the test fair as we are investigating resistance. The
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What Factors Affect the Period of a Pendulum? Introduction In this experiment‚ my group will find out what are the factors that affect the period of a pendulum. In this report‚ I will personally explain the aim of the experiment‚ the hypothesis of my group‚ the variables‚ the apparatus‚ method‚ data‚ data analysis‚ evaluation and last but not least the conclusion. Aim To find out whether the heavy or light pendulum will affect the period as well as the string’s length Hypothesis
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How Does Temperature affect the Ability to Bounce of a Ping-Pong ball? Physics Design Practical Research Question How is the bounce-height of a ping pong ball affected by it’s temperature? This research questions aims to find out how the temperature of a ping pong ball affects the vertical height bounce‚ after being dropped from 1 metre. I will be measuring the bounce height using a video camera to find the heightest point. I will use the top of the ball for ease of results. Hypothesis:
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CHAPTER – 29 ELECTRIC FIELD AND POTENTIAL EXERCISES 1. 0 = Coulomb 2 Newton m kq1q2 2 =l M L T 1 –1 –3 4 F= 2. r2 3 q1 = q2 = q = 1.0 C distance between = 2 km = 1 × 10 m kq1q2 r 2 so‚ force = F= (9 10 9 ) 1 1 (2 10 ) 3 2 = 9 10 9 2 2 10 6 = 2‚25 × 10 N 3 The weight of body = mg = 40 × 10 N = 400 N So‚ 2.25 10 3 wt of body = 4 10 2 force between ch arg es 1 = (5.6) –1 = 1 5 .6 3. So‚ force
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Preparation and melting point of Aspirin Aspirin is a painkilling drug and is produced worldwide to reduce and stop pain. Aspirin is made up of ehtanoylation of 2-hydroxbenzenecarboxylic acid in a presence of phosphoric acid. AIM: The aim of the experiment is to prepare and produce aspirin as pure as possible. I will do this threw preparing the aspirin and purifying the product threw recrystallization. I will then obtain a melting point of my product to get an estimate of its purity. By the end
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Circuit Variables 1 Assessment Problems AP 1.1 To solve this problem we use a product of ratios to change units from dollars/year to dollars/millisecond. We begin by expressing $10 billion in scientific notation: $100 billion = $100 × 109 Now we determine the number of milliseconds in one year‚ again using a product of ratios: 1 year 1 hour 1 min 1 sec 1 year 1 day · · · = · 31.5576 × 109 ms 365.25 days 24 hours 60 mins 60 secs 1000 ms Now we can convert from dollars/year to dollars/millisecond
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How the Addition of Extra Sugar Affects the Growth of Plants Lacey Fish Florence Nguyen Danielle Wang Julia Wu Botany 10MWF April 4‚ 2006 Materials: 1. 20 Mung beans 2. 4 plastic cups 3. soil 4. Turbino sugar 5. Distilled water 6. Beakers 7. Graduated cylinders 8. Stir plate 9. Scale 10. String 11. Ruler with cm measurements 12. Stir bar Background: We chose this project because we were curious to see how the amount of raw sugar
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Paper Cone Investigation Lisa Han 10MF Design Research Question: This investigation will determine how the sector sizes of the piece of the filter paper affect the terminal velocity for the cone to fall. The following will be kept constant meter ruler‚ stopwatch‚ and filter paper. Hypothesis: ‘If the sector size is the increase‚ then the terminal velocity will decrease.’ The reason is that if the sector size is bigger‚ the surface area of the cone will become smaller. If the surface area
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Exam-type questions on Thrust Bearing design 1. You have been asked to design a minimum power-loss‚ uni-directional‚ thrust bearing for an horizontally-mounted‚ axial‚ ammonia compressor in a nitric acid plant. The compressor has a shaft diameter of 100 mm and generates a thrust of 15kN when operating at 6‚000 rpm. The thrust is negligible at zero speed. a. What type of thrust bearing would you recommend and why? b. What are the physical reasons why a dry‚ PTFE bearing would
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