Introductory Biochemical Laboratory Worksheet 10 - DNA Melting Curve Course Code: BICH172 Course Name: Introductory Biochemical Laboratory Student Name: CHUNG YUK MAN EMILY Student ID: 10198465 Group Number: 10 Date of Experiment: 16th November 2010 Q1. State the experimental aim and describe the term “hyperchromic effect”. (2 mark) The experimental aims are to study the basic structural characteristic of DNA molecules through UV spectrum and thermal denaturation
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When DNA profiling was first used in criminal cases‚ it was often difficult for prosecutors and defense attorneys‚ as well as the experts they hired to testify‚ to explain the significance of their DNA match to the jury. Fingerprints are still considered by most people to be an ironclad way to identify someone‚ but an expert testifying about fingerprints discusses them in terms of "points of similarity." DNA matches are discussed in terms of statistical probability using what is currently known about
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procedure for extracting DNA‚ collecting DNA samples‚ and to observe the physical characteristics of DNA Background: The wild strawberry is a diploid because it only has two sets of chromosomes. While the grocery store kind is an octoploidy because it has eight sets of chromosomes. The reason we chose the grocery store strawberry is because we’d be able to extract more DNA. Ripe strawberries make enzymes which help break down the cell walls making it easier to extract the DNA. We will be using a solution
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DNA Extraction from Wheat Germ and Making an Agarose Gel AIM: To be able to make and agarose gel and perform gel electrophoresis in six different dyes. Also‚ to extract DNA from wheat germ. INTRODUCTION: Agarose gel is a substance that is used in science for gel electrophoresis and size exclusion chromatography. These processes use agarose gel to separate and analyze proteins and DNA. The medium is composed of a purified agarose powder that has been boiled in a buffer solution and then cooled
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1. Outline the Steps of DNA: a. DNA Replication begins at the Origin of Replication b. Helicase cuts hydrogen bonds and separates DNA in half c. Semiconservative replication produces two copies that each contained one of the original strands and one entirely new strand. d. Topoisomerases catalyze and guide the unknotting of DNA e. Single Strand Binding Proteins attach to the halves and keep the DNA molecules separated (they are needed because the sides are attracted to each other and with out
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Biology 110 March 2‚ 2015 DNA Lab BACKGROUND In this laboratory experiment‚ students were introduced to DNA electrophoresis. DNA electrophoresis is an instrument that many forensic scientists use to get a DNA fingerprint as an evidence for crimes. Not only can it be used for forensic science‚ people can use this for paternity test‚ as well as look for evolutionary relationships among organisms. Agarose is used to make the gel that the DNA fragments are going into. Since DNA particles are negatively
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Extraction of DNA from an Onion Molecular biologists and biochemists are involved with research in finding out as much as possible about the DNA in plants. DNA was discovered in the 1950’s‚ there still remains a lot to be known about it‚ especially how it is used to determine the physical traits that we all have‚ and how it regulates the workings of the body. deoxyribonucleic acid is a chemical‚ we can do reactions with it just like we can work with any other chemical. Experiment: Note:
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DNA Deoxyribonucleic acid DNA Deoxyribonucleic acid DNA is a nucleic acid that is basis of genetic information. It is like a set of instructions for our bodies and genetic codes. DNA has many different components that make it up such as hydrogen‚ oxygen‚ nitrogen‚ and phospurous. The structure has two long polymers of a simple unit called nucleotides. Holding the back of the structure is sugar and phosphate which are attached by ester bonds. Bonded to each sugar is 4 different types
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instances of how our knowledge of DNA has changed life in our day. Give one positive and one negative application of that knowledge and defend your answer. According to Kenneth Saladin‚ deoxyribonucleic acid (DNA) is “a long threadlike molecule with a uniform diameter of 2mm‚ although its length varies greatly from the smallest to the largest chromosomes.” What does this mean? Basically‚ it is a long string that contains a person’s genetic information. DNA has been studied overtime and it
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DNA Analysis The importance of DNA analysis has made an impact the forensic world by proving the innocence or guilt of a person. DNA analysis is the most effective method in the Forensic Investigation field‚ it carries more evidentiary value than other forensic evidence‚ it has become a primary source for cases that have not been solved and the advancements in technology will continue to scientifically eliminate wrongful convictions. Although many feel that there are discrepancies in DNA analysis
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