which the absorbance is the at the greatest. [pic] INTRODUCTION A spectrophotometer or colorimeter makes use of the transmission of light through a solution to determine the concentration of a solute within the solution. A spectrophotometer differs from a colorimeter in the manner in which light is separated into its component wavelengths. A spectrophotometer uses a prism to separate light and a colorimeter uses filters. Both are based on a simple design of passing light of a known wavelength through
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Purity of Aspirin Objectives - To research‚ using various sources‚ the history of aspirin‚ its use in medicine‚ methods of synthesizing it and of measuring its purity - To compare the % purity of a branded aspirin tablet with a generic aspirin tablet - To compare 2 methods of composition analysis of the two types of aspirin Research
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Day one consisted of cutting the beetroots and placing them at each temperature while day two was used to measure how much green light was absorbed by the colorimeter. This could have affected the results because light could have reached the tubes causing some of the pigment to be damaged and not give accurate results. There are not many solutions for this but I suggest that we perform the lab experiment with
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which differs greatly from the specification. Possible sources of this error are * Using a measuring cylinder instead of a volumetric flask when diluting * Contaminated test tubes * 2. If this analysis was performed using a colorimeter‚ yellow light would be used to measure the absorbance of solutions. Why is blue light not used? Blue light cannot be used to measure absorbance of the solution because it cannot be absorbed. Instead of absorbing‚ the solution reflects blue light
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The amount of light that is scattered is equal to the number of cells. A colorimeter may also be used to determine the absorbance much easily and accurately. This would be at around 400nm – 600nm. A haemocytometer can also be used to count the number of microorganisms present in a culture. This is not the best method to use because
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BCBT: As it can be seen in table 2‚ E. platyloba EO had a strong activity in maintenance of β-carotene molecules especially in C10 (78.25%)‚ which was higher than ascorbic acid and comparable with BHT. The same concentration of ascorbic acid and BHT were used as standard to compare the values. FRAP: The antioxidant efficiency of the E. platyloba EO using FRAP method was calculated with reference to the reaction formula ‚ given by a Fe+2 solution of known concentration. The same concentration of ascorbic
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(though special probes are sometimes used to measure the pH of semi-solid substances). A typical pH meter consists of a special measuring probe (a glass electrode) connected to an electronic meter that measures and displays the pH reading. A colorimeter is a device used in colorimetry. In scientific fields the word generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. This device is most commonly used to determine the concentration
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ethanol oxidation through the LoggerPro System. This data then helped determine the kinetic rate constant‚ k‚ and the order of the reaction. First‚ the wavelength of maximum absorbance was determined using the LoggerPro interface and a Vernier colorimeter. Beer’s law was then used to determine the molar absorptivity. Finally‚ a kinetic study of ethanol oxidation was completed through the kinetic run on the LoggerPro interface. Chemical kinetics is the study of chemical reactions in relation to reaction
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Implementation My aim for this experiment was to find the effect of temperature on the membrane permeability of beetroot. Since I was unfamiliar with this experiment‚ I first conducted a preliminary test. I considered my preliminary test as my trial for this experiment. My Hypothesis for this experiment was to found out the amount of pigment loss of beetroot when exposed to varying temperatures. Betalain pigment found in beetroot My method for this experiment is:- * Use a cork borer to cut about
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Discussion: The effect of wavelength of light on the rate of photosynthesis was investigated in this experiment. The results from Figure 13 suggest that white light‚ containing all wavelengths of coloured light‚ has the highest rate of photosynthesis. Purple light had the second highest efficiency‚ followed by blue‚ red‚ and yellow light. Green light was significantly less efficient‚ producing the second lowest pH reading (Figure 14). The samples exposed to no light had the lowest rate of photosynthesis
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