pH 8 where there is a steep increase from 30% to 39% as the enzyme has reached its optimum pH at 8. During the experiment in the boiling tube this pH had the deepest red colour as the most protein gelatine was broken up. This meant that in the colorimeter when testing to see what the light transmission‚ this pH let the least light through. For this enzyme as the pH increases the percentage of light transmission decreases until it gets to pH 8 where it increases again to 39%. From the graph the
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5cm³ of distilled water‚ one in each of the water baths to bring them to the required temperature. Place a beetroot section in each boiling tube and leave for 30 minutes. Set up the colorimeter for 490 nm light absorption‚ and 2/3rds fill cuvettes with water from each of the 8 boiling tubes. Calibrate the colorimeter and put in the cuvettes‚ taking care to place them so the light passes through the smooth sides‚ and record the readings. Repeate the readings for all 8 temperatures to check results
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Biology IA: Quantitative Estimation of Sugars to Soft Drinks Trials Data Collection and Processing: Percentage Transmissions of Light through a Glucose Solution after a Benedicts Treatment | Trial 1 | Trial 2 | Trial 3 | Trial 4 | Trial 5 | Trial 6 | Trial 7 | | Glucose Concentration | Transmission (%)± 0.1 | Transmission (%)± 0.1 | Transmission (%)±0.1 | Transmission (%)± 0.1 | Transmission(%)±0.1 | Transmission (%)±0.1 | Mean (anomalous data not included) ±0.1 | Standard Deviation
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5vol (0.42moldm-3) hydrogen peroxide (H2O2). For the second part of my investigation‚ the KI solution will remain a constant 0.3moldm-3 and the H2O2 solution will vary. H2O2 + 2I- + 2H+ -> 2H2O + I2 Methods to find the rate: 1 - Use a colorimeter to monitor the change in absorbance as the reaction progresses‚ showing how the concentration of Iodine changes with time and how varying concentrations of KI and H2O2 solutions affects the rate of reaction. 2 - Use an iodine clock: add 2cm3 of
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absorption occurs in the "green" region of the spectrum in both graphs 1 and 2. Graph 2 shows two peaks‚ one in the violet region (chlorophyll a) and one in the yellow-orange region at around the wavelength 670 nm. Due to the limitations of the colorimeter and its incapability of recording light absorbed by chlorophyll in between wavelengths of 600-700 nm‚ we did not have a recording for this region and thus no peak is shown. Also‚ in graph 1 ‚ there is more absoption of yellow light while in graph
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An investigation to measure the percentage light transmission‚ using a colorimeter‚ through a solution‚ from pH 2 – pH 9‚ in which jelly cubes were immersed over a 24 hour period Interpretation Written Communication of the Data C1 Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that
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You will choose one factor and investigate this factor’s effect on the rate of reaction between sodium thiosulfate and dilute hydrochloric acid. A colorimeter is a device used in colorimetry that generally refers to the device that measures the absorbance of particular wavelengths of light by a specific solution. The output from a colorimeter can be shown by an analogue or digital meter and may be shown as transmittance (a linear scale from 0-100%) or as absorbance (a scale from zero to infinity)
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Investigating the effects of temperature on cell membranes Independent variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency
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decreased‚ and therefore the amount of pigment released from the beetroot samples is increased. The results produce a graph with negative correlation‚ a curve that has a negative gradient. The graph shows that as the temperature is increased‚ the colorimeter reading is decreased. The structure of my results proves my theory correct. The increase in
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absorbance. As the reaction between the chemicals takes place and the Crystal Violet receives the hydroxide the overall intensity of the purple color will decrease thus affecting the absorbance. The absorbance of the solution will be measured with a colorimeter as the reaction takes place and will be interpreted as a direct representation of concentration of Crystal Violet. After the reaction has taken place‚ through analysis of graphs plotting absorption vs. time‚ the natural log of absorption vs. time
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