University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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In fermentation the pyruvate is converted into ethyl alcohol‚ through the oxidation of the 2 NADH molecules‚ which returns them to two NAD+’s (Freeman‚ 2011). Oxidation is the loss of an electron in this case H+. We used information from previous labs in which we tested
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Introduction There are about a million different kinds of seeds in our world with all of the different types of plants that we have. One type of seed that takes between three to five days to germinate is a daikon radish. The radish (Raphanus sativus ) originated from China. It was commonly seen and eaten by the people of Egypt long before the Egyptians started to construct their very own pyramids. Later on‚ radishes became a common vegetable to eat in Greece‚ and since the radish meant so much worth
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Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor
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Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen
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for human erythrocyte behavior because permeability similarity increases for more closely related species (Hunter 1976). Using an even more closely related species’ erythrocytes in diffusion experiments would provide an even better model for human cell behavior. Additionally‚ finding out more about diffusion mechanisms may allow scientists to synthesize compounds that have specific desired diffusion characteristics. This will allow better targeting of drugs‚ which would have a great impact on medicine
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tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure: 1) Preeminently‚ follow all laboratory safety guidelines ensuring that you have safety goggles on at all times throughout the lab. 2) Fill the cuvette 2/3 of the way with water. 3) Select the appropriate wavelength on the spectrophotometer. 4) Place the cuvette in the spectrophotometer and click the calibrate button. 5) Put 5mL of stock solution into test tube. 6) Fill
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Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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Student Name: Amaan Rushdi Lab Partner Name: Dylan Course: BIOL 123 Lab Instructor: Megan Grandinetti Sowbug behavior on environment -lab report What area does the sowbugs prefer to choose for their dwelling? Abstract By working with physical isopods‚ ordinarily known as pill bugs‚ sow bugs or roly- polies‚ we tried whether these sowbugs favored a dark spot to a light place. Included in the lab are a few outlines and tables sketching out our aftereffects of the test. We measured recurrence
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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