DNA FINGERPRINTING Background Reading - Nelson Biology and Campbell Biology Purpose - To understand the basics of DNA fingerprinting used in the Canadian courts for crime convictions and paternity suits. Introduction The process of DNA fingerprinting was developed by Professor Alec Jeffreys at Leicester University in 1984 as a form of genetic analysis. It was first used in the law courts of England in 1987 to convict a man in a rape case. It has now been used successfully in many crime and paternity
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Erin Bolton Chemistry Lab Report April 29‚ 2015 Lab: Reaction Rates Introduction: In this experiment we studied the reaction of potassium persulfate‚ K2S2O8‚ with potassium iodide‚ KI. All chemical reactions have an energy barrier to overcome before the reaction will proceed. We will record data based on the concentration‚ temperature and catalyst for each experiment. Once this has been completed it will be graphed. Procedure: Due to the chemicals being used having hazard gloves are used
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Observing a Single Chemical Reaction Qualitative vs. Quantitative Observations Abstract: The purpose of this experiment is to help you sharpen your observational skills. The aluminum foil was found to rust in the water containing the dissolved Copper Chloride (CuCl₂) crystals. The results of the experiments were determined through close examination and observation of both qualitative and quantitative elements of the changes that occurred when a blue crystal‚ Copper Chloride‚ reacted with aluminum
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin‚ and carbonic anhydrase) will be placed into separate wells within the gel‚ and the apparatus
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very useful because of its ability to detect a specific DNA sequence from a large amount of DNA ⎯ even from the whole genome. This specific sequence can be found through a combination of two different techniques: Agarose Gel Electrophoresis and Hybridization‚ which is known as Southern Blotting. This technique if performed in three phases: (I) prepare the gel and (II) make the blot‚ (III) hybridize and visualize blot. In phase one‚ the gel is prepared in three steps: (1) chemical digestion‚ (2)
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DNA Profiling and Ethics Reflection Journal Vanshika Khemka 14th October 2012 "33 autorad off" On 10th September 1984‚ geneticist Alec Jeffrey’s wrote these three words in his red desk diary. This marked the completion of an experiment‚ which studied how inherited illnesses pass through families. The experiment failed entirely. (McKie‚ 2009) However‚ this led to the most profound discovery: the world’s first DNA fingerprint. Now‚ the smallest swab of blood or sweat can determine
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of steel wool into the test tube and measured the temperature again. We stored the test tubes on the test tube rack to observe the next day. A chemical reaction did occur in the lab. There were a color change and an odor change that was observed on Day 2 of the lab. On Day 1‚ the liquid was clear and the smell was very strong. On Day 2 of the lab‚ most of the liquid was gone but the color was a muddy color and the smell got milder‚ it wasn’t as strong as Day 1. Not only was there a color and odor
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Determining Allele Frequencies of the PV92 Alu Element using DNA Isolated from Human Cheek Cells and PCR Amplification Background Alu elements are the most abundant repetitive elements in the human genome that have mobilized throughout primate genomes by retrotransposition over the past 65 million years ago from a 5’ to 3’ fusion of the 7SL RNA gene‚ to reach the present number of more than one million copies. Over the last few years‚ several lines of evidence demonstrated that these elements
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Your first lab link can be found at Case One: Rookie Training. 1. What are the four basic steps of DNA processing? The four basic steps of DNA processing are extract‚ amplify‚ separate‚ and analyze. 2. From the toxicology lab‚ what is vitreous humor? Why do forensic scientists use it? Vitreous humor is the clear liquid inside the eye that gives the eye its shape‚ forensic scientists use this to confirm results received from the blood or urine. 3. From the survey in the toxicology lab‚ would you
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