experiment was broken into three sections. The first section showed the pH and color changes that an enzyme can create. It was predicted that when potato extract‚ the enzyme‚ was added to catechol‚ the substrate‚ an enzymatic reaction would occur. The second section of the experiment demonstrated enzyme specificity. It was predicted that potato extract‚ when hydroquinone was introduced‚ would not exhibit the characteristics of an enzymatic reaction. The third section of part one focused on whether
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Therefore‚ it will be presumed that the result of this experiment by isolating the polyphenol oxidase within the potato extract through the oxidation of 5mM dopa solution with highly concentrated enzymatic preparation of polyphenol oxidase with a buffer as the diluent will indicate a rapid increase in the rate of the reaction as well as the amount of activity present in the potato extract. Proposing that Vmax is proportional to enzyme concentration‚ a linear relationship should be present and enzymatic
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Pre-lab: look up the structure of lycopene. Introduction: Lycopene is the red pigment in ripe tomatoes and‚ as an antioxidant‚ helps to fight certain cancers. In this lab you will isolate lycopene from tomato paste. To do this you will first extract carotenoid pigments from the paste and then use column chromatography to isolate the lycopene from the other pigments. You will then use TLC to evaluate the column chromatography separation. Note: because lycopene is light-sensitive‚ prevent any
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pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results. We will answer question such as what is the best temperature for optimum
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separated in this lab contains three components: naphthalene‚ C10H8‚ common table salt‚ NaCl‚ and sand‚ SiO2. The separation will be done according to the scheme shown on the next page and involves three basic steps: 1. Heating the mixture to sublime the naphthalene. 2. Dissolving the table salt with water to extract. 3. Evaporating water to recover dry NaCl and sand.2 Mixture: naphthalene‚ NaCl heat to naphthalene
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a sodium chloride (NaCl) solution‚ subjecting the product to detergent solution (dH2O)‚ filtering the solution and lastly‚ the addition of ethanol. When beginning with a solid substance‚ such as a banana‚ crushing the substance allows for breaking down the cell membrane and cell wall‚ if using a plant cell. This ultimately‚ removes the contents of the cell from the deoxyribonucleic acid (DNA) and further exposes it for the detergent solution. After this step‚ one would use NaCl solution to stabilize
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Nov 19/12 Solute Concentration of Potatoes Block 2-2 Purpose: To determine the concentration of solute in the potato’s cytoplasm by measuring the change in mass after the process of osmosis. Materials and Equipment: Refer to Biology 12 Lab Manual – Investigation 13 Procedure: Refer to Biology 12 Lab Manual – Investigation 13 Data and Observations: The Potatoes change in mass after the process of Osmosis Test Tube # | Concentration
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was added to the funnel and shaken to mix the layers. The lower organic phase was drained into the Erlenmeyer flask and the aqueous phase out of the funnel. After that‚ the organic layer was returned to the separating funnel and washed with 15% NaCl solution. Anhydrous sodium sulfate was used as the drying agent and allowed the solution to stay for 15 minutes. The Erlenmeyer flask was weighed and the pipet was used to transfer the solution from the drying agent to the flask. Finally‚ the hood
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values (10‚ 7‚ 6‚ and 3) Change in color of the solution The amount of potato extract‚ pH solution‚ and catechol used (1 cm +/- .1cm) Size of the test tubes Amount of time allowed for the catechol to sit with the potato extract and pH solution (20 minutes with 5 minute intervals for observation) Materials: 4 test tubes Test tube rack Wax pencil Small ruler Buffer solutions of pH 10‚ 7‚ 6‚ and 3 Potato extract Catechol Procedure: 1. Using the small ruler and the wax pencil‚ three
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Abstract: Enzymes‚ molecules that speed up chemical reactions‚ are specific to one substrate. In this experiment the substrate hydrogen peroxide and the enzyme catalase will be used. The higher the concentration of potato extract‚ or catalase‚ the faster the reaction and the more substrate present will result in a decrease in the time of the reaction. The amount of concentrations of enzymes and substrates are changed to determine if the reaction is further catalyzed by a greater concentration of
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