University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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It is responsible for sending oxygenated blood throughout the body as well as sending deoxygenated blood to the lungs‚ and continuously circulate this way for as long as we are alive. Many things can be effected‚ for example how fast the heart beats or how much force the heart chambers contract with. In this experiment‚ many different methods will be used to test the effects of both external electrical stimulus as well as injected reagents on the heart rate and force of contractions of a frog.
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Kathleen Kramas Cells and Heredity Lab Tuesday 2:00-4:50 22 OCTOBER 2012 Membrane Permeability Decreases as Molecular Size Increases Introduction: Red blood cells are vital to organisms functioning properly. They are microscopic cells that carry oxygen from the lungs to all the tissues throughout the body. Upon transporting oxygen‚ red blood cells also exports waste‚ such as carbon dioxide‚ to the lungs where it can be expelled. Red blood cells are made up of hemoglobin which is surrounded by a
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OBSERVING WAVE PULSES - EXPERIMENT 15 PART 1 - Transverse Wave Pulses Q1 - As the pulse travels down through the spring‚ its‚ size and amplitude is reduced - the sizes of the waves are much smaller as the forces become weaker (losing energy) as the distance from the point of initiation is increased. Q2 - Pulses do not ’catch up’ with one another in transverse waves. They either meet at a certain point momentarily or pass through each other and continue on their way as if nothing has happened.
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Introduction In unit 7.3 the experiment tested the ability of lactase to specifically bind and interact with lactose compared to maltose. In unit 7.4 the experiment tested the role‚ if any‚ that metal ions have on the activity of lactase. My hypothesis for unit 7.3 was knowing that lactase is specific for lactose‚ lactose will separate into galactose and glucose‚ as maltose will not change (153-155). Lactase should like lactose. For unit 7.4 my hypothesis was that EDTA will remove the ions‚ and
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Limiting Reagent and Percent Yield Aim To determine the limiting reagent between the reaction of lead (II) nitrate and potassium iodide. To determine the percent yield of lead (II) iodide. Date Started: 13/4/12. Finished: 19/4/12. Data collection and processing Measurements: * Amount of distilled water: 75.0ml ± 0.5ml. * Mass of watch glass: 31.65g ± 0.01g. * Mass of watch glass + potassium iodide: 32.45g ± 0.01g. * Mass of potassium iodide: 0.8g ± 0.02g. * Mass of watch
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EXPERIMENT I Photoreduction of Benzophenone Introduction The study of chemical reactions‚ isomerizations and physical behavior that may occur under the influence of visible and/or ultraviolet light is called Photochemistry. The fundamental principles for understanding photochemical transformations are that light must be absorbed by a compound in order for a photochemical reaction to take place‚ and that for each photon of light absorbed by a chemical system only one molecule is activated for
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Chemotherapeutic Agents of Control: Introduction: Chemotherapeutic agents are chemical substances used to treat various forms of infectious diseases. The chemotherapeutic agent works by stopping the bacteria from reproducing. There are two different kinds of chemotherapeutic agents. The first kind is an antibiotic‚ which slow down the growth of microorganisms. The second kind of chemotherapeutic agent is synthetic drugs. Synthetic drugs are artificially made in a laboratory. Chemotherapy
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Data collection Quantitative Data Raw Data Table 1: Table showing the mass of the amount of unknown acid X measured in grams (±0.001g) Table 2: Table of reading of the burette initially filled with 25mL of 0.201moldm-3 sodium hydroxide (NaOH) to titrate 25mL (±0.03mL) of unknown acid X in mL (±0.05mL) after each titre. Reading on the burette initially filled with 25mL of 0.201moldm-3 NaOH (±0.05mL) First titre 21.3 Second titre 18.2 Third titre 15.2 Fourth titre 12.0 Qualitative
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ENG 4UI: ISU Essay Outline ----------------------- Introduction: All life and existence makes an unavoidable journey towards an ultimate end. Immortality is a mythical unattainable existence‚ barred by the presence of entropy. Mortality is a constant found in everything that was‚ is and will be. Hook:
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