Effect of different temperatures on the rate of an enzyme controlled reaction I will place starch and amylase into five water baths which are at different temperatures‚ and record the time it takes to break down the starch in the solution. Independent variables The independent variable is what I am going to change in my experiment. In this case it is the temperature of the water in the five water baths- 10‚ 25‚ 40‚ 55‚ 70 degrees Celsius Dependant variable This is what will stay the
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Effect of Concentration‚ pH and Temperature on Enzyme Activity Biology For Majors October 4‚ 2012 Abstract We examined the reaction an enzyme has when its concentration‚ pH and temperature are altered. In order to do this‚ we added different levels of pH into different test tubes with the enzyme (sucrose)‚ and substrate (sucrose)‚ and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature increases
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solution of KMnO4 and H2SO4‚ the solution will turn colourless and it will take a specific time for the reaction to complete. By increasing the temperature‚ the time it takes (the rate) for the solution to completely change to colourless will be increased. Thus it can be said‚ that it is being hypothesized that an increase in temperature will cause an equal increase in the rate of the reaction. Method:1). Using a measuring cylinder‚ place 50 cm3 of sulphuric acid into a 250 cm3 beaker‚ add 50 cm3
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To investigate the effect of different caffeine concentration on the heartbeat rate of Daphnia at room temperature. Introduction: Caffeine is found‚ and produced in many plant species to function as a natural pesticide‚ and as an inhibitor of seed germination of other nearby coffee seedlings to give it a better chance of survival. Caffeine is able to paralyze and kill certain insects upon feeding on the plants that produce caffeine. Coffee‚ tea and cocoa are common caffeine-containing
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makes them highly specific and only work on one or a few similar chemical reactions. Enzymes themselves are not consumed in the reaction‚ but they help attract substrates into correct position to undergo chemical reaction. Enzymes greatly speed up the rate of biological reactions by lowering the energy of activation. To get a sense of the speed and efficiency of enzymes‚ substrates can be transformed to products at the rate of thousands of times per second with the enzymes. If we consider the total
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Thermochemistry Thermochemistry is the study of heat evolved and absorbed during the chemical reactions. The system is the interest of the universe; the surroundings are the rest of the universe in which the exchange of the energy with the system takes place. Both‚ the system and the surroundings make up the universe. Heat flow is the transfer of heat from a warm place to a cooler one. System to surroundings: Surroundings to system:
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environments affecting the rate of reaction‚ PNPP (p-nitrophenyl phosphate) + H20 ? PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase‚ which alters its active site‚ and thus changes its efficiency to catalyze the reaction. We measured the rate of reaction‚ by using a chromogenic
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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