The pH is another important factor that influences the enzyme activity‚ as it affects the shape of the enzyme or charge on substrate and ultimately its functionality (Reece et al.‚ 2011; Eed and John‚ 2013). With respect to effect of pH on enzyme activity‚ there was a steady increase in the activity for CFR15-protease‚ i.e. from pH 3.5 to 10.5 and a sudden fall was observed over pH 10.5. The activity was stable in the range of pH 7 to 10.5 and this reveals the alkaline protease nature of the enzyme
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2.5 10-5 M 4.9 10-5 M 7.0 10-3 M 3.7 10-2 M 5. What is the pH of the buffer that results when 12.5 g of NaH2PO4 and 22.0 g Na2HPO4 are mixed and diluted with water to 0.500 L? (The acid dissociation constants for phosphoric acid are Ka1 = 7.5 10-3‚ Ka2 = 6.2 10-8‚ and Ka3 = 3.6 10-13) (Points : 10) 2.30 7.04 7.38 12.27 12.62 6. Which of the following combinations would be the best to buffer an aqueous
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Marble-lous Momentum Question: What happens to momentum when one marble collides with other marbles? Hypothesis: If a marble collides with increasingly more marbles‚ then the momentum will be transferred more slowly to the final marble‚ because momentum is conserved through an inelastic collision‚ but a longer distance will have be traveled by the kinetic energy. Independent Variable: The number of marbles. Dependent Variable: The momentum of the first marble and final marble. Control Variables:
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Q1) Describe and compare BOTH the similarities and differences between spatial and temporal summation Summation can be defined as the method which neurons use to communicate with each other‚ determining if the cumulative postsynaptic potential in the neuron is enough to pass the threshold for firing. There are two types of summation‚ spatial summation and temporal summation. Both types of summation are similar in that they work to strengthen the EPSP of a neuron‚ meaning they help create more postsynaptic
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3A Task 1 Aim: the aim of the experiment is to find the best temperature to ferment yeast at. Hypothesis: the yeast will ferment the best at 60 degrees Celsius. Independent variable: the temperature of the water the yeast is put in to ferment. Dependent variable. The amount of air bubbles the yeast produces. Controlled variable: the amount of yeast and glucose in each syringe. Uncontrolled variables: human error in counting. Materials : Plastic soft drink bottle cut to size Marking pen
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on the rate of reaction. By using self investigative and experimental skills‚ the experiment was done in order to determine how the rate of reaction will be altered‚ whether it will increase‚ decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant‚ animal‚ and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical
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Hypothesis: I hypothesis that the enzyme concentration will affect the rate of reaction but only up until a certain point because there is a limited supply of substrate. Enzymes are protein molecule that acts as biological catalyst by increasing the rate of reactions without changing the overall process. They are long chain amino acids bound together by peptide bonds. Enzymes are seen in all living cells and controlling the metabolic processes in which they converted nutrients into energy and new
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Effect of Temperature on Solubility of a Salt Ahmed Mohammed 17-nov-2013 Abstract In this experiment‚ you will study the effect of changing temperature on the amount of solutet will dissolve in a given amount of water. In this experiment‚ you will completely dissolve different quantities of potassium nitrate‚ KNO3‚ in the same volume of water at a high temperature. As each solution cools‚ you will monitor temperature using a computer-interfaced Temperature Probe and observe
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Spectrophotometric Determination of the Equilibrium Constant of a Reaction DEPARTMENT OF MATERIALS‚ METALLURGICAL AND MINING ENGINEERING‚ COLLEGE OF ENGINEERING July 26‚ 2012 ------------------------------------------------- ABSTRACT ------------------------------------------------- Before proceeding to test the next solution‚ we must first clean the cuvette‚ using similar steps earlier. First‚ we rinse with water‚ then with the solution‚ and then we fill it up and test
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purpose of laboratory assignment 3 was to measure the rate at which a chemical reaction takes place. For the purpose of this lab we measured the rate a balanced oxidation/reduction reaction between iodine‚ hydrogen‚ and bromate ion occurs. The above reaction occurs slowly so we used a coupled iodine clock reaction to measure the rate of the oxidation/reduction reaction because it occurs much faster but is still dependent upon the other reaction. To accomplish this‚ two mixtures were prepared in separate
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