product mixture of ferrocene and Acetylferrocene 2. To separate and purify the components in the product mixture by column chromatography. 3. To check the purity of the components by thin-layer chromatography (TLC). 4. To calculate the yield of acetylferrocene and the percent recovery of unreacted ferrocene. Table of Quantity showing various physical properties Type of substance Molecular Formula Molecular Weight (g/mol) Density(g/cm3) M.P.(oC) B.P.(oC) Solubility Ferrocene C10H10Fe 186.03
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Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for
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Zebracki Organic Chem II Lab 2/15/15 Friedel-Crafts Acylation of Ferrocene and Column Chromatography Introduction: The intention of this lab is to analyze the formation of acetylferrocene using column chromatography. The Friedel-Crafts acylation reaction created acetylferrocene and diacetylferrocene‚ using phosphoric acid as a catalyst for the reaction between acetic anhydride and ferrocene (once applying heat). During column chromatography‚ a solution is passed through a filtration system of silica
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Separating Ferrocene and Acetylferrocene by Adsorption Chromatography Separating Ferrocene and Acetylferrocene by Adsorption Chromatography Dry Pack Method Leah Monroe February 27‚ 2003 Organic Chemistry Lab II Experiment performed on February 18 and 20‚ 2003 Abstract: Adsorption column chromatography is one way to separate compounds out of a mixture. In this technique‚ a solid stationary phase called the adsorbent is packed in a glass column and an eluent‚ which is the mobile phase‚ moves
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Acetylation of Ferrocene 17. October 1996 Experiment #7 Introduction In this lab we will be utilizing the Friedel Crafts process of acetylation of ferrocene. Ferrocene is an atom of iron bounded by two aromatic rings. We will use some reagents that will cause the ferrocene to add either one acetyl group to an aromatic ring or add two acetyl groups to each of the aromatic rings. In order to determine how well this process had worked we employed: IR spectra analysis‚ column chromatography‚ and a little
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Tittle: Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis
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Visayas State Unversity Visca‚ Babay City‚ Leyte Name: Millama‚ Engene O. Course & Year: BS Biotech-2 Lab Schedule: W (1-4) Group 5 Date Performed: Dec. 12‚ 2012 Date Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll
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The Separation of Ink Chromatography Emanuel Alvarado Period 06 Group Members: Jason Fernandez‚ Reyna Favela & Lucero Ochoa I. Title: The Separation of Ink Using Chromatography II. Date: October 3rd‚ 2012 III. Purpose: To separate a mixture using paper chromatography. IV. Procedure: 1. Fill beaker with 100 mL water. 2. Poke hole in filter paper with scoopula approximately 1 cm from top. 3. Place dot of ink approximately 2 cm from the bottom of the filter paper
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Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as
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Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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