3.6.5. Southern hybridization Southern blot hybridization analysis was carried out using standard procedures (Sambrook et al. 2001). Approximately 20 µg of total genomic DNA from transgenic plants and untransformed control plant was digested with EcoRI‚ and then separated by electrophoresis on 0.8% agarose gel. Digestion profile was checked with 1µg DNA‚ with single cutter enzymes EcoRI (10U) in T-DNA region. For southern experiment‚ 20µg DNA was digested overnight. Restricted fragments were separated
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Southern Pulp and Paper Introduction In this work I am going to identify the Southern Pulp and Paper’s Toccoa mill problems and issues that are available to mill’s manager Curtis Shelton. Part 1 In considering the overall process on the pulp and bleached products industry it is always good to start with the main performance objectives and measures. One of the first important issues is the process design and layout. How the process architecture looks like and how well it is designed
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Female Characters in Gothic Texts Gothic fiction has become a popular area for feminist studies. Many commentators have noticed how females in Gothic fiction often fall into one of two categories: the trembling and innocent victim or the shameless and dangerous predator. However‚ others have noticed how women writers have often used the Gothic to explore aspects of femininity and sexuality. The mad woman in the attic in Jane Eyre has become a key symbol of Gothic feminism. The persecuted maiden
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China Southern Airlines was founded in 1995 and is based in Guangzhou. It is China’s largest airline in terms of fleet size‚ with 309 aircraft. The airline employees over 45‚500 full time employees. Its main hubs include Guangzhou and Beijing‚ but it operates in over 150 cities throughout China. Furthermore‚ China Southern operates 616 domestic 82 routes‚ including 90 international routes and 25 routes in Hong Kong and Macau. As China’s only company that is in world top ten passenger airlines
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Capitol of Panem. In the end of the games‚ both Katniss and Peeta win after many many trials. When they win President Snow is very angry at Katniss because there is only supposed to be one winner. This movie is a great example of a Gothic literature. Gothic literature is a genre that combines fiction‚ horror‚ death‚ and romance. The five elements that need to be
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means to have a Southern accent‚ your mind goes straight to the stereotypical renditions you have heard from media portrayals of the South. Probably‚ the examples you think of are highly exaggerated‚ like Scarlett O’Hara’s Deep South‚ r-less rendition of a Southerner exclaiming Well ah nevah! (Bailey 11) or like those in “Gone with the Wind” or “Dukes of Hazzard County‚” both of which reinforce the negatively stigmatized‚ stereotypical Southern accent (Lippi-Green 218). Southern American English
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south is known for a lot of things‚ amazing sweet tea‚ good barbeque‚ but have you ever thought about southern hospitality? No‚ it isn’t one of Ludacris’s albums‚ well‚ not in this sense anyway. Southern Hospitality to me is defined as people being willing enough to volunteer their time‚ homes‚ churches‚ etc to the community‚ or even country. Tennessee in particular is well known for their southern hospitality‚ and willingness to volunteer. We’re called the Volunteer State because in the War of 1812
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the south because of its ability to grow in the Chesapeake soil. The intense physical labor required of southern famers led to the popularization of indentured servitude as a cheap supply of labor. The desire to invest in cheap labor inhibited any mature forms of settlement‚ instead farmers‚ even the wealthy‚ usually slept in primitive housing consisting of tents and shanties
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In the fifth chapter‚ this thesis will assess the abolitionist effort to denounce the legitimacy of using the Bible to sanction southern slavery by arguing that biblical slavery was not based upon the inferiority of one race whereas southern slavery was based upon the inferiority of one race. In short‚ these abolitionists sought to highlight that southerners were using a book which sanctioned a system of slavery that was not based upon the inferiority of one race to sanction a system of slavery that
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This particular novel: The strange case of dr jakyll and mr hyde contains a lot of Gothicism in it which is portrayed throughout the play. Gothic writing has a tendency to be very dark‚ focuses on the evil side of human nature‚ have supernatural and unexplained factors to it‚ and feature numerous suspense and thriller. All of those may be seen in the book The Strange Case of Dr. Jekyll and Mr. Hyde. This book focuses on the darkness which means that it specializes in frightening‚ mysterious‚ depressing
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