THE EXTRACTION OF DNA THE EXTRACTION OF DNA 07/02/2013 | By Laura Green‚ Tasnim Rahman and Sandra Strammiello | Table of Contents DNA………………………………………………………………………………………..………… 2 The Discovery of DNA…………………………………………………………………………...…………..…………. 4 The Steps for Extracting DNA…………………………………………………………………………………………….…..… 5 Experiment: Which Product Can the Most Visible Amount of DNA Be Extracted From?..............................................................................................
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highest DNA yield over all of the fruits at 1.93%. Our next highest DNA yield was at 1.11% and came from our blueberry sample. Although our blackberry had the third highest DNA yield there was a significant drop in our percentage to .921%. Based on our results the banana had the least amount of DNA yield with .138%. Discussion The purpose of this experiment was to be able to better understand the properties associated with DNA in live organisms and to find out how to isolate and quantify DNA in plant
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For the preparation of the filtrate solution at the beginning of the class our Instructor‚ Marshall Douglas added about four to five strawberries in the blender along with 100 ml of detergent/ enzyme/ salt solution. They were blended until thoroughly homogenized if foaming occurred more detergent solution was added to prevent it. Then the blended solution was poured into a beaker and left to incubate at 60C for 15 minutes. Also‚ the mixture solution was cooled on ice for 5 minutes and filter through
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temperature‚ nothing would work out right. Once we got the alcohol perfectly chilled‚ we mixed it with the sweet smelling strawberries‚ and our concoction was complete. But we still screwed up. The kid we were trying to impress just ran screaming right out of the lab. Earlier‚ my partner and I explained to the kid that we would be extracting DNA from a strawberry‚ and that this same DNA stuff was in everyone’s body. But I guess it was too much for him to handle because as soon as the white gunk formed
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E3 Title Osmosis in Living Tissue Catalytic Activity of Enzyme Action of Electricity on Substances Encountered in Daily Life Do All Conductors Obey Ohm’s Law? Composting Bioreactor Sunglasses’ Protection from Ultraviolet (UV) Radiation Extracting DNA from Fruits How Cross-linking Changes the Properties of a Polymer Integrated Science Sample SBA Task Practical Related Task – C1 Osmosis in Living Tissue Student Handout (Version 1) Purpose To determine the water potential of potato tissue. Background
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Lab manual/results/online article condensed notes Lab 1 · DNA is made up of deoxyribonucelotides · Components of DNA/RNA includes a 5 carbon sugar‚ a phosphate group‚ and a nitrogenous base · the negative charge associated with DNA/RNA is due to the phosphate groups · DNA is linked together by phosphodiester bonds (they are covalent bonds) · the energy required to create these bonds is from the cleavage of pyrophosphate‚ refer to figure 1.3 on page 4 and below. ·
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breeding individuals with similar characteristics B.DNA Technology DNA finger printing (gel electrophoresis) analysis of DNA sections that vary between individuals procedure i.restriction enzymes cut DNA into fragments at specific base pair sequences electrical current moves through DNA through gel Fragments separate by size: shortened DNA move farther than long. Every one banding pattern is unique Uses: forensics Comparing DNA sample fro crime scene it suspects victim paternity test diagnose
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Cloning plants Many plants clone themselves naturally to reproduce. They send a small shoot-like structure called a runner‚ along the soil. The runner grows into a new separate plant‚ which is genetically identical to the original plant - a clone. People can clone plants by simply taking a cutting of the plant such as a twig or stem and planting it. This is called vegetative propagation. Horticulturists use cloning to grow plants with specific qualities‚ like height‚ flower colour and quality
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and are located in the cytoplasm‚ Rough ER‚ and Mitochondria. Golgi Apparatus: Dionysus‚ or as the campers call him‚ “Mr. D‚” is in charge of the strawberry service the camp runs and the weapons that the campers make. He processes the swords and makes sure they are fit to send outside of the camp‚ and controls the exporting of the strawberries‚ just as the Golgi Apparatus processes lipids and proteins before they exit the cell. Chloroplasts: The Kitchen/Dining pavilion is where the food is
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should GM foods be implicated in the rise in allergies? GM critics believe that the inherent cross-species nature of biotechnology may be responsible. GM foods are created by splicing genes from the DNA of one organism into the DNA of another possibly unrelated organism. In experiments‚ strawberries have been spliced with fish genes‚ rice and tobacco with human genes and even lettuce with rat genes. Since genes are the instruction codes for proteins‚ and proteins are implicated in allergic reactions
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