Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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Lysergic Acid Diethylamide (LSD) was created by chemist Albert Hofmann in Switzerland while attempting to create a blood stimulant. The drug was utilized for psychological experiments for many years and this even led to the military experimenting with it as a biological weapon in the 1960’s. During the 1960’s‚ as a result to the wide dispensing of the drug for experimental purposes‚ it wound up being the “…counterculture used the drug to escape the problems of society…” (LSD: A Short History). Due
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In “Helping Students Use Textual Sources Persuasively‚” Margaret Kantz talks about how individuals should read texts to write a synthesis essay. To write a synthesis essay‚ one should be able to come up with an argument and find information from other sources‚ and use them as proofs to back up the argument. First‚ Kantz talks about the mistakes that students usually make when they write this essay. For instance‚ Shirley made a mistake by reading a repeating and telling the story instead of creating
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Indications | Contraindications | Drug Effects/Interactions | Adverse Rxns | Unique | Beta1 Adrenergic Sympathomimetics | Dopamine/Intropin (neurotransmitter/catecholemine) | * Dopamin is the only receptor to stimulate these site * A precursor in synthesis of NE * Action: Beta agonist/alpha agonist- dose dependentDopaminergic | * Dilate renal blood vessels‚ brain‚ mesentery and heart vessels increased blood flow * Stimulate alpha/beta receptors/vasoconstrictor‚ increase CO2 improves contractility
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April Goodson CHEM 242L-002 February 20‚ 2013 Oxidation of Cyclohexanone to Adipic Acid Abstract The cyclic ketone cyclohexanone was oxidized to adipic acid using the oxidizing agent nitric acid. The experiment yielded 0.2667 grams of adipic acid‚ giving a percent yield of 113.97%. Although the product was allowed to dry for one week‚ residual moisture was still present in the sample and a melting point could not be obtained. This error in the experiment either resulted from adding too much
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Description of reagents. Reagents Description Salicylic acid Fine powdery white solid crystals 95% ethanol Clear colorless liquid Acetyl chloride Clear colorless liquid with gas escaping from container FeCl3 Clear colorless liquid Commercial aspirin Fine powdery white solid crystals I2/KI Deep black liquid KMnO4 Brownish liquid Phosphoric acid Clear colorless liquid Table 2. Preparation of Aspirin. Description Salicylic acid + Acetyl chloride Cloudy white liquid with undissolved
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In the reaction mechanism‚ the alpha hydroxyketone’s hydroxylic group attacks the positively charged nitrogen from nitric acid attaching it to the molecule. The nitrogen’s electrons are then kicked off on to the oxygen bearing a positive charge‚ creating water. This process formed an organic nitrate. Next‚ water is used to abstract hydrogen from the positively charged oxygen‚ forming hydronium. A carbonyl is formed as well as nitrogen dioxide gas. Simultaneously the oxygen separates itself from nitrogen
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medulla oblongata as well as airway obstruction may cause respiratory acidosis. 3. Explain how the renal system would compensate for respiratory acidosis. The kidneys regulate the amount of H+ ions and HCO 3- excreted in the urine which regulate acid base balance. A C T I V I T Y 3 Renal Responses to Respiratory Acidosis and Respiratory Alkalosis 1. Describe what happened to the concentration of ions in the urine when the PCO2 was lowered. How well did the results compare with your prediction
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Calcium Oscillations: Synthesis and Binding Studies of Photo-reversible Calcium Chelator Harpreet Sandhanwalia December 2‚ 2011 Dr. Alison McCurdy Abstract: Living bodies maintain a homeostasis by constant communication amongst the various cells and organs. Calcium plays a very important role in controlling this network of communication and henceforth helping in carrying out many essentials functions. However the exact working of Calcium
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The Role of DNA in Protein Synthesis DNA is found in the cells nucleus. It is part of the chromosome of the cell. DNA is the molecule that controls the making of protein. Protein is used for the growth of cells and organisms and also for the repair of cells. Protein is also used as an enzyme to speed up reactions in the body. Because of this DNA is able to control the whole organism. With out DNA and its ability to make protein the cells and eventually the whole organism would die
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