The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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Secondary excitotoxicity is thought to be more relevant to chronic neurodegenerative disorders than the classical acute form. In this pathway increased glutamate levels are not required as disturbances with the neuronal resting membrane potential cause glutamate receptors to be over-activated by physiological levels of glutamate. In a neuron which is affected by another pathological process and shows reduced ATP production‚ Na+ dysfunction or impaired glucose metabolism and as a result has an abnormal
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The movie Juno is fiction and came out in 2007. It is about Juno MacGuff a 16 year old high schooler who gets pregnant after her first time having intercourse with Paulie Bleeker‚ who is also in high school. Although 9 years old‚ this movie is still extremely relevant in today’s society in regards to teen pregnancy. It tackles all the different options one can take and how emotionally‚ physically‚ and mentally challenge this decision is‚ especially when you’re a teen. Psychologist David Elkind theorized
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The nonmutated form of DMPK functions in the production of protein kinase serine; this enzyme plays a role in intracellular communication and in the regulation of myosin phosphate. When DMPK is mutated‚ the proteins CUG-BP1‚ MBNL1 and INSR are displaced causing the splicing of troponin pre-mRNA which causes malfunctions in the cardiac conduction system‚ dominance of a chloride channel that causes myotonia‚ dominance of an insulin receptor that affects the sufficiency of insulin‚ and dominance of
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Introduction: The pharmacokinetics of a drug describes the journey of a drug through the body i.e. its absorption‚ bioavailability‚ distribution‚ metabolism and excretion. Opioids are substances (endogenous or synthetic)‚ which produce ‘opiate-like effects’. Opiates are alkaloids derived from the Papaver somniferum poppy e.g. Morphine‚ Codeine (a morphine-like agonist) and Methadone (a synthetic analogue of morphine) and are primarily used as analgesics.[1] Discussion: Morphine has a 4-ring structure
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NARRATOR: Once upon a time there was a beautiful young girl named Cindelila. She had a cruel stepmother. STEPMOTHER: And…did you make breakfast? CINDELILA: Yes‚ breakfast is ready. STEPMOTHER: Did you iron my clothes?? CINDELILA: Yes‚ I did. STEPMOTHER: Did you clean the kitchen??? CINDELILA: Yes‚ way to finish… STEPMOTHER: Uhhh-OHHH!! I see dirt!! Here….Here….Here…and you yourself!!!!! IS THIS WHAT YOU CALL CLEAN??? GET FAR AWAY FROM ME U THIS DIRTY GERM!!! EWWWWW!
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In passing‚ the correlation between color and status is very important. As mentioned above‚ even though Irene Redfield is African-American‚ she displays zero signs of empathy and hires maids of her own race. Irene believes that she is superior to her workers and looks down on them. Larsen describes Zulena as ‘mahogany‚’ comparing her to furniture. “Zulena‚ a small mahogany-coloured creature‚ brought in the grapefruit.” (Wilson 979). Larsen’s interpretation of Irene’s treatment toward Zulena and the
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purpose of a synthesis essay is to make insightful connections. Those connections can show the relationship(s) between parts of a work or even between two or more works. It is your job to explain why those relationships are important. In order to write a successful synthesis essay‚ you must gather research on your chosen topic‚ discover meaningful connections throughout your research‚ and develop a unique and interesting argument or perspective. A synthesis is not a summary. A synthesis is an opportunity
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Introduction The American Chemical Society databases were investigated through Scifinder Scholar Version X to find any information about Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate. There was no previous information present. Then‚ the microscale synthesis and characterization of Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate was conducted with the base catalyzed Knoevengael condensation of the butyl cyanoacetate and corresponding aldehyde (gkharas/235_14F/235_14F_Lab_2.pdf) and analysis. The elemental
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Protein Synthesis Worksheet 1. 2. a. Protein synthesis began in the nucleus. b. Chromatin is a diffuse mass located in the nucleus where genetic material is found. c. A gene is a sequence of nucleotides‚ it is important because they form the template and these templates are used for synthesizing proteins. 3. a. Transcription is the first step of protein synthesis it uses a sequence of DNA nucleotides to produce a sequence of RNA nucleotide. b. RNA polymerase bonds to RNA nucleotides together
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