Salivary Amylase Domingo‚ Guray‚ Hugo‚ Lorenzo‚ Mohammad Isa Intro Because everything has a start Catalysis The process of increasing the rate of reaction with the use of a catalyst. Catalyst – any substance that increases rate of reaction upon addition to a certain reaction Page 3 Enzymes Act on substrates in a reaction Highly specific Breaks down complex macromolecules‚ synthesizes compounds essential for the cell Active site Enzyme-substrate complex
Premium Enzyme PH
Effects of pH on fungal amylase activity BI 211 November 25‚ 2011 Introduction In recent years‚ the uses of microorganisms have become a huge importance to industry and sparked a large interest into the exploration of enzyme activity in microorganisms. Amylase is one of the most widely used enzyme required for the preparation of fermented foods. Apart from food and starch industries‚ in which demand for them is increasing continuously‚ amylase is also used in
Premium PH Enzyme Starch
information regarding the effects of varying the concentration of a substrate‚ in this case the pea extract. To view the graphical representation‚ which more clearly displays the relationship‚ view Figure 2. The rate at which the reaction occurred for the 100% concentration is 1.45 mg/dL per minute. For the 50%‚ the rate was 3.05 mg/dL per minute‚ and for the 25%‚ the rate was 2.76 mg/dL per minute. As seen‚ the rate fluctuated from the lowest rate at 100% and the greatest rate occurring at 50%. As mentioned
Premium Chemical reaction Chemistry Reaction rate
Total CO2 Evolved by Different Concentrations of Convollaria roots. Actual values are the graduated pipette readings. For CO2 evolved values‚ subtract the initial reading from the actual reading. This is the amount of CO2 accumulated over time. | Time (min) | Tube 1 | Tube 2 | Tube 3 | Tube 4 | | Actual | CO2 Evolved | Actual | CO2 Evolved | Actual | CO2 Evolved | Actual | CO2 Evolved | | (A) | (A-I) | (A) | (A-I) | (A) | (A-I) | (A) | (A-I) | Initial reading (I) | 0.05 | | 0
Premium Yeast Bacteria Metabolism
are catalysts meaning they speed up the rate of the reaction. Enzymes activity depends on the concentration of the substrate‚ temperature and the pH. The more concentrated the substrate is the more reactive the enzyme is. The optimal pH for an enzyme is 7.5 and the optimal temperature for an enzyme is 53 Celsius. Extremes in the temperature and the pH of an enzyme can denature therefore destroy it. The enzyme that is in this experiment is Amylase. Amylase is found in germinating seeds. These seeds
Premium Enzyme Chemical reaction Catalysis
the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants‚ animals and microorganisms. Large amounts of amylase occur in germinating cereals‚ and in the pancreas and saliva of higher animals. Aim The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures. Hypothesis As the reaction temperature
Premium Temperature Energy Thermodynamics
increase the rate of reaction without being used up by themselves. They are found in and out of cells and lower the activation energy of a reaction. Amylase is the enzyme which catalyses starch hydrolysis. Alpha amylase and beta amylase are two types of amylase enzymes. The amylase which is the most commonly found in the human body is the alpha amylase. Beta amylase is mainly found in bacteria‚ fungi and plants. Amylase breakdown starch into maltose. During the process of hydrolysis‚ Amylase degrades
Free Enzyme
pH Affecting on Amylase Enzyme Is there a difference in the concentration of pH in the amylase enzyme activity? The null hypothesis would be that there will be no difference in the concentration of pH and alternative hypothesis would be that there will be a difference in the concentration of pH. The predication if that there will be a difference in the concentration of pH. The pH affects on enzyme activities‚ one of the enzyme that was used in the process was amylase. When a serial dilution was
Premium Enzyme Starch Chemistry
The Effect of pH on Enzymatic Reaction Rate Abstract Enzymes are affected by changes in pH. The most favorable pH value— the point where the enzyme is most active—is known as the optimum pH. This experiment was conducted to determine the effect of pH reaction rate on an enzyme‚ catalase‚ from yeast. The experimental results indicate that the catalase worked best at a neutral pH level of seven (7). Introduction An enzyme is a protein molecule that serves as a catalyst. “The basic function of
Premium Enzyme Chemical reaction Catalysis
tested the effects of temperature on fungal amylase and bacterial amylase (Aspergillus oryzae and Bascillus Licheniformis). We used 4 different temperatures in Celsius 0‚ 23‚ 58‚ and 89 for both fungal and amylase. For 10 minutes‚ every 2 minutes we would use 3 drops of each amylase and mix it with iodine to observe the presence of starch at each temperature. We conducted this experiment for both bacterial and fungal amylase. Results were reached based on the color of the iodine and amylase mixture
Premium Enzyme Starch Amylase