Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life’s processes would not come so easily. Enzymes are basically proteins‚ which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape‚ which makes enzymes different from other proteins. A common enzyme that we have is catalase‚ which breaks down
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Title: Investigate the breakdown of starch at different temperature AIM This experiment has been done to investigate the action of the enzyme amylase on the breakdown of starch. MATERIALS These were the materials used: four starch/ agar plates‚ a marker pen‚ 1mm graph paper ruler‚ 8mm cork borer‚ forceps and template for cutting holes‚ 1% Amylase‚ water‚ incubator set at 5‚ 20‚ 40 and 60 degree Celsius. METHOD This was the experimental procedure carried out: the materials above were collected
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Part B: Practical Report The Effect of Temperature on Enzyme Activity Aim: To investigate how temperature effects the enzyme catalase. Hypothesis: If the temperature of water is increased then the enzyme will react quicker to form oxygen and water‚ when compared to cold water. Purpose: To design and conduct a plan of a practical about the effects of temperature on enzymatic activity with a partner. Introduction: An enzyme is a protein‚ which speeds up a specific chemical reaction without altering
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Chemical Kinetics of Hydrogen Peroxide Decomposition Purpose The purpose of this experiment was to determine the rate equation for the catalyzed decomposition of hydrogen peroxide H2O2. Procedure The procedure for this lab can be found on pages 36 - 40 in “General Chemistry CHE111L Laboratory Manual Spring 2014.” Data Tables Carbon copies of data tables and the graph have been attached to the back of this lab report. Calculations 30 mL of
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involving hydrogen peroxide and catalase. Tests were performed by putting chicken liver‚ ethanol solution (diluted ethanol solution for other trials) and hydrogen peroxide in a test tube with a side arm‚ and having a rubber tube lead the oxygen gas into a gas collection tube. Results from the tests showed a negative correlation‚ this means that the more diluted the solution of 95% ethanol was‚ the less oxygen gas collected. Controlling a number of factors which include human error‚ temperature change
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In my coursework I will investigate about enzymes in potatoes reacting with Hydrogen Peroxide. In particular I will investigate the effects of changing the surface area of a potato when added to Hydrogen Peroxide. This is because‚ when increasing the surface area of the potatoes it will increase the rate of reaction because there will be more surface area on which particles from the potato and the Hydrogen Peroxide will collide on‚ and with more surface area there would be more particles carrying
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Assessment of Catalase Function Lab Introduction The purpose of this lab report was to test and measure the rate of substrate destruction by an enzyme‚ we tested the destruction of hydrogen peroxide by the enzyme catalase. Hydrogen peroxide is a poisonous by product of metabolism that can damage cells if it is not removed. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water and oxygen gas. H2O2 + catalase → H2O + O2 A catalyst is a substance that lowers the
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Abstract In this laboratory exercise we studied enzyme catalase‚ which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the
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that Affect the Rate of Reaction of the Decomposition of Hydrogen Peroxide Emilio Lanza Introduction- In this experiment‚ the rate of reaction‚ calculated in kPa sec-1‚ of the decomposition of hydrogen peroxide will be investigated to see how the change in concentration of hydrogen peroxide and the change in temperature affect the rate of reaction. The data will be collected by measuring the gas pressure. The product of Hydrogen Peroxide is oxygen in a gas state thus it is mandatory to use the
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