water (120 ml) and stir vigorously to assist the hydrolysis of unreacted acetic anhydride. After 30 min‚ the oil which first separates will gradually solidify.* Collect the crude product using Hirsch funnel and record the crude yield of the product. Filter‚ wash well with cold water and re-crystallize several times from hot methanol/water (3:4) mixture until the mp is constant. Record the melting point. *If solidification is not resulted: Dissolve the oily residue in hot methanol/water (3:4) mixture
Premium Acetic acid Carboxylic acid Nuclear magnetic resonance
Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
Premium Catalase Chemical reaction Catalysis
sure it is properly suspended. Take dropper to pick up sample material. Put a small amount on the glass slide. Take a cover glass by the corners with tweezers to not get any finger prints. Put cover from corner down to avoid air bubbles. If too much water use a tissue or filter paper to suck up remaining water. Part B: 1. Linear measurements: measure the width of your textbook in cm and then convert to mm. 2. Conversions: convert 100 grams to mg and then ug 3. Weight measurements: using your scale
Premium Microscope Optics Lens
fluid containing benzene to clean the production-line machinery that fills bottles for North America. Perrier now finally admits that benzene naturally occurs in Perrier water but the problem was now visible because the workers forgot to replace the filter. Then Perrier faced a big problem.
Premium Water Carbon dioxide Mineral water
themselves from germs and bacteria. In the other study of Reyniers’ experiment of a bacteria free animal‚ Dunn introduces the anecdote of the bubble boy. The bubble boy was a child who “had been transferred antiseptically into a chamber at birth because he lacked an immune system” (76). Dunn uses this one instance of the bubble boy to prove that the bubble boy died after reattachment to other germs and bacteria due to his lack of interaction with these species. Together these experiments are anecdotes
Premium Into the Wild Jon Krakauer Nature
Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
Premium Osmosis Cell membrane Red blood cell
Separation of a Carboxylic Acid from a Neutral Compound by Extraction Reference: Smith‚ Chapter 2 (Acids and Bases) Introduction Carboxylic acids and phenols are two families of organic compounds that contain carbon‚ hydrogen and oxygen‚ and also react with water to yield an excess of hydronium ions over hydroxide ions. Pure water has a pH of 7‚ which means it has a hydronium ion concentration‚ [H3O+] of 10-7 M (M = molarity‚ moles/Liter). The hydronium ions in pure water come from the self-ionization
Premium Acid Acetic acid PH
1. INTRODUCTION 1.1 GENERAL Water recycling or the reuse of treated wastewater is becoming a necessity in today’s world. Under constant threat due to climate change‚ explosive population growth‚ and wastage‚ there is tremendous pressure on water‚ a basic and valuable resource. While industrial growth and unplanned water utilization for agricultural use have depleted ground water levels in many parts of India‚ the growth of urban clusters have brought pressure on available water. Increasing
Premium Sewage treatment Wastewater
Project Title: Examination of bacterial content of sea water samples from selected sites in South East England Introduction Over the past few years coastal beaches across the UK has seen lavished with recognition for their cleanliness‚ by sections of the media and various organisarions. Majority of these beaches have been awarded the Blue flag award in recognition for meeting set criteria on various standard including cleanliness‚ water quality‚ environmental management and environmental education
Premium Bacteria Microbiology Water
Materials and Methods Growing the G Strain and Preparing the GCE (rGFP Crude Extract): To grow the bacterial culture‚ use 10 ml of liquid LB growth media for incubation. 500 ml of the bacterial culture is allowed to grow overnight at 37°C. It is later shaken vigorously to increase the OD600 to 0.5‚ which means that time equals zero. At time zero‚ 1 mL of the culture is transferred into a 1.5 mL centrifuge tube and centrifuged for 5-10 minutes to obtain a pellet. The supernatant should be discarded
Premium Western blot Molecular biology Gel electrophoresis