rubber gloves food chopper (food processor or blender) 12 - quart-size wide mouth glass canning jars w/lids large funnel w/strainer built in (fruit strainer) 1 gallon glass jug w/tight-fitting screw-on cap funnel big enough to hold a coffee filter coffee filters electric hotplate heat spreader pan to use as double boiler cooking thermometer 500 ml Pyrex flask w/one-hole stopper small ice bucket or plastic container large plastic tub Liebig condenser bag of ice water circulation pump potholder or cooking
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water (120 ml) and stir vigorously to assist the hydrolysis of unreacted acetic anhydride. After 30 min‚ the oil which first separates will gradually solidify.* Collect the crude product using Hirsch funnel and record the crude yield of the product. Filter‚ wash well with cold water and re-crystallize several times from hot methanol/water (3:4) mixture until the mp is constant. Record the melting point. *If solidification is not resulted: Dissolve the oily residue in hot methanol/water (3:4) mixture
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cylinder to the bag with the fruit it in. Seal the bag completely. Mix well by kneading the bag for two minutes. Create a filter by placing the center of the cheesecloth over the mouth of the standing test tube‚ pushing it into the tube about two inches‚ and securing the cheesecloth with a rubber band around the top of the test tube. Cut a hole in the corner of the bag and filter your extraction by pouring it into the cheesecloth. You will need to keep the filtered solution which passes through the
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Running head: LAB 5: INVESTIGATING AN ENZYME-CATALYZED REACTION Lab 5: Investigating an Enzyme-Catalyzed Reaction September 24‚ 2014 Principles of Biology 120.601 Mrs. Annemarie Duncan Abstract: (Burmania) This experiment was performed in order to examine ways in which a potato catalase enzyme reacts to various assays with differing variables. To do so a baseline assay (undiluted extract and room temperature H2O2) was used within the experiment with only one other variable changed in
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sure it is properly suspended. Take dropper to pick up sample material. Put a small amount on the glass slide. Take a cover glass by the corners with tweezers to not get any finger prints. Put cover from corner down to avoid air bubbles. If too much water use a tissue or filter paper to suck up remaining water. Part B: 1. Linear measurements: measure the width of your textbook in cm and then convert to mm. 2. Conversions: convert 100 grams to mg and then ug 3. Weight measurements: using your scale
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fluid containing benzene to clean the production-line machinery that fills bottles for North America. Perrier now finally admits that benzene naturally occurs in Perrier water but the problem was now visible because the workers forgot to replace the filter. Then Perrier faced a big problem.
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themselves from germs and bacteria. In the other study of Reyniers’ experiment of a bacteria free animal‚ Dunn introduces the anecdote of the bubble boy. The bubble boy was a child who “had been transferred antiseptically into a chamber at birth because he lacked an immune system” (76). Dunn uses this one instance of the bubble boy to prove that the bubble boy died after reattachment to other germs and bacteria due to his lack of interaction with these species. Together these experiments are anecdotes
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Separation of a Carboxylic Acid from a Neutral Compound by Extraction Reference: Smith‚ Chapter 2 (Acids and Bases) Introduction Carboxylic acids and phenols are two families of organic compounds that contain carbon‚ hydrogen and oxygen‚ and also react with water to yield an excess of hydronium ions over hydroxide ions. Pure water has a pH of 7‚ which means it has a hydronium ion concentration‚ [H3O+] of 10-7 M (M = molarity‚ moles/Liter). The hydronium ions in pure water come from the self-ionization
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Laboratory Report The Plasma Membrane I. Introduction The Plasma membrane is the edge of life‚ the boundary that separates the cell from its surroundings. It controls the traffic of materials in and out of the cell. (Reece‚ 2011). It is incredibly thin that is very vital in maintaining the integrity of the cell. Not only does the plasma membrane bind the other organelles‚ it also forms a dynamic structure which gives them their remarkable activity and selectivity. (Hickman‚ 2008). Diffusion
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1. INTRODUCTION 1.1 GENERAL Water recycling or the reuse of treated wastewater is becoming a necessity in today’s world. Under constant threat due to climate change‚ explosive population growth‚ and wastage‚ there is tremendous pressure on water‚ a basic and valuable resource. While industrial growth and unplanned water utilization for agricultural use have depleted ground water levels in many parts of India‚ the growth of urban clusters have brought pressure on available water. Increasing
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