LABORATORY REPORT Activity: Effect of Dietary Fiber on Transit Time and Bile Name: Hope Schallert Instructor: Dr. Weithop Date: 03.08.2015 Predictions Dietary fiber will: increase transit time Dietary fiber will increase the amount of bile acid in solution Materials and Methods Effect of Dietary Fiber on Transit Time 1. Dependent variable. number of radiopaque markers in GI tract 2. Independent variable. amount and type of fiber in meal 3. Controlled variables. calories in meal‚ nutrients
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Introduction Enzymes are key players in metabolism. A metabolism is the organic processes in a cell or an organism that are necessary for life. An enzyme affects the rate at which a reaction occurs when the activation energy is lowered. In this reaction the reactant is called the substrate which is that combine with enzymes molecules to form a temporary enzyme substrate complex. During this products are formed and the enzyme molecules released is unchanged. For the substrate complex to form the
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Physiology Enzyme Lab Report Introduction The chemical reaction that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a form of energy and would
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experimental group had a huge impact when the diluted concentration of caffeine was put in compared to the control group that contained no concentration of caffeine. The hypothesis was supported because a significant decrease of the total number of cells when caffeine solution was added to the experimental group was clearly shown. An addition of small amounts of caffeine does inhibit cell proliferation and affect cell viability during the lab experimentation one more test was conducted because the
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Solute Concentration on the Mass of Apples Introduction Background: This lab involves the testing and measuring of water potential. Water potential (ψ) is the tendency of water to diffuse from one region to another. It measures the free energy available in water to do work. Pure water has a water potential of 0 (kpa--unit?). Water will move from an area of higher water potential to an area of lower water potential. Several factors‚ including temperature‚ pressure (ψp) and solute concentration (ψs)
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The effect of increasing substrate concentration on rate of an enzyme reaction. Enzymes are biological catalysts that lower a reactions activation energy making possible many of the reactions needed for life to exist. Enzymes have a high specificity which have been explained by many theories such as Fischer’s lock and key. Currently the most widely accepted theory is the induced fit hypothesis proposed by Koshland in 1958. This hypothesis solves some of the problems with the Lock and key theory
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Starch is a type of Carbohydrate that’s made from thousands of glucose units. Simple sugars are the basic units that make up starch. Carbohydrates provide us with energy so that we can carry out our daily routines. Our body then digests it into glucose so we can have energy to do that. Saliva is a form of chemical digestion that is in the mouth. Amylase is an enzyme that catalysts the breakdown of starch into sugars. Amylase is present in human saliva‚ where it begins the chemical process of digestion
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Strengths of Materials Lab #4 CIVENG 303 Section 804 Stress Concentrations Performed October 24th‚ 2013 Today’s Date: November 7th‚ 2013 Tim Hendrickson OBJECTIVE The objective of this lab is to determine a set of changes due to the stress in geometric irregularities in an axially loaded bar. Some of the irregularities include certain features such as holes and notches. QUESTIONS: -The following are formulas are for stress and strain that are biaxial. Sample Material
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Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The data recorded in (table 27) and (figure 29) illustrate the effect of temperature of the
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Sample Lab Report: Factors which Affect the Activity of the Enzyme Catalase Purpose: Must include: background information about concepts involved in the lab‚ statement of purpose of the lab identification of independent and dependent variables. A hypothesis is often not necessary or appropriate. Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzyme has only one substrate that it breaks down. Enzymes are produced in
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