Reaction Rates of Barley Alpha Amylase with Starch at Specific pH ’s and Temperatures Page 1 Abstract Enzyme ’s are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest‚ any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum
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An Investigation on the rate of reaction of the enzyme Catalase on the substrate Hydrogen peroxide. Plan Aim: To investigate the rate of the effect of Catalase on hydrogen peroxide. Introduction This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide. Enzymes are biological catalysts‚ which speed up the rate of reaction without being used up during the reaction‚ which take place in living organisms. They do this by
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The effect on rate of an enzyme catalysed reaction by different objectives which include effect of substrate‚ temperature‚ ph and effect of a competitive inhibitor phosphate ions. This is determined by the reaction of hydrolysis by p-nitrophenylphosphate (PNP) as a substrate by the enzyme phosphatase. Abstract The hydrolysis of p-nitrophenyl phosphate has been studied in human red blood cells. To see if hydrolysis was related to the functioning of the sodium pump. Acid phosphatase catalysis’s
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An experiment to investigate the effect of temperature on the rate of reaction of the Enzyme Trypsin. Aim: This investigation was on the effect temperature has on the rate that the enzyme trypsin hydrolyses its substrate‚ a protein found in milk (casein). This investigation was conducted under controlled conditions‚ the temperature being the changeable variable. Trypsin and its substrate (powdered milk which is a source of the protein casein) were heated in a water bath. The contents of the two
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trial produced the most oxygen of all three trials. Heat tends to increase the rate of chemical reactions‚ explained in the article Temperature Effects (Introduction to Enzymes) by Chris Jamison. “Like most chemical reactions‚ the rate of an enzyme-catalyzed reaction increases as the temperature is raised. A ten degree rise in temperature will increase the activity of most enzymes by 50 to 100% . Variations in reaction temperature as small as 1 or 2 degrees
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Hypothesis: If I increase the concentration of NaOH from 0.5M to 1M‚ then I will see the temperature slope increase in the reaction with an increased concentration because with an increase of moles‚ the particles will be colliding more often‚ therefore increasing the probability that the proper energy and alignment will occur to create more collisions in the same amount of time. Methods: List of Materials: • 110 mL of NaOH at 1 M (10 mL per run) • 110 mL of NaOH at 0.5 M (10 mL per run) • 220 mL
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An investigation to show how the rate of reaction between hydrochloric acid and sodium thiosulphate is affected by the concentration of the acid Simple Procedure Place a conical flask on a piece of paper with a cross on it. Add hydrochloric acid and sodium thiosulphate‚ and record the amount of time taken for the cross to disappear through the solution from the top of the flask. Record this time and repeat this for different concentrations of hydrochloric acid. Fair Test The variables in
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found that‚ in acidic pH environment‚ the reaction rate of starch being broken down by alpha amylase is less than that of the reaction rate at a neutral and slightly basic pH environment. This finding partially supports our hypothesis. The spectrophotometer readings in our experiment measured the absorbance of 3-amino-5-nitrosalicylic acid‚ a colored molecule formed after dinitrosalicylic acid (DNSA) has reacted with the products of the enzymatic reaction or the simple sugars. Therefore‚ the absorbance
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VARYING EFFECTS OF ENZYME CONCENTRATION ON REACTION RATES OF MALATE DEHYDROGENASE CELL BIOLOGY 13 NOVEMBER 2007 Enzymes are biological catalysts. They are proteins that speed up reactions with low concentrations. These enzyme proteins are made up of linkages of amino acids. The links coil‚ and coil again forming a tertiary structure. This structure has a groove in it called an active site. The active site is
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Aim: This experiment will aim to show how the amount of substrate affects the rate of the reaction. Hypothesis: In this experiment I think the amount of substrate will simply increase the reaction. As I increase the surface area of the potato the gas given off from the reaction will increase. Therefore to sum things up‚ my hypothesis is when the Independent variable increases so will the dependant variable. Independent variable: - Surface area of potato Dependant variable: - Gas Controlled
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