Title: DNA analysis Aim: a) Isolate and Purify Bacterial Chromosomal DNA from a strain of E.coli b) Visualization of restriction fragments by Agarose Gel electrophoresis Objectives: * to isolate and purify bacterial chromosomal DNA from a strain of E.coli * to analyze and identify DNA by use of a spectro-photometer * to use restriction enzymes to cleave DNA into fragments * to visualize the restriction fragments by gel electrophoresis * to compare the different
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Mtunzini (Mm-tun-zee-nee) is a small coastal town that is situated almost exactly halfway along KwaZulu-Natal’s coastline in South Africa approximately 140 km north of Durban. The name is an isiZulu word meaning place in the shade. After the breakup of the Zulu Kingdom after the Anglo-Zulu War‚ Sir Garnet Wolseley created 13 ’kinglets’ - with two strategically located as buffer zones between Port Natal and Zululand. One of these kinglets was John Dunn who used Mtunzini as his capital. Umlalazi
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Poetry Unit (A): “Ordinance on Lining Up” “Waiting hurts. Forgetting hurts. But not knowing which decision to take can sometimes be the most painful.” –Jose N. Harris. Choosing the poem that appealed to me the most turned out to be a pain in the neck. We make countless decisions like this every day‚ and although this is not a life-changing decision‚ we have all at some point in our lives come across a difficult and stressful situation. The wide range of connections and the accuracy of
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Heaven as I see it is not above the Earth. It is an ever expanding planet at the end of space. Even though it is a great distance from Earth‚ one just has to look skyward as if looking into a massive telescope and will see the far reaches of space. Gigantic planets and clouds of different colored gases and lights are as easily seen as the faces of your loved ones back on Earth. Every morning starts with a sunrise that is more intense and awe-inspiring as the one the day before. During the day there
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“A Process of Breaking Down” Julia Greenburger 1/9/13 The Crack up‚ an essay by F. Scott Fitzgerald‚ is an elaborate description and analysis of the mental break down the author experienced and the depths of its causes. After a life of struggling to succeed and failing multiple times‚ he suddenly reconsiders why he needs to succeed at all and whether it is what he really wants. Fitzgerald’s breaking down was a process of losing all
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India a traveler’s heaven‚ a woman’s hell: American student IANS | Aug 22‚ 2013‚ 11.00 AM IST comments Share More A A READ MORE Chicago University|Sexual Harassment|Michaela Cross|Female Tourists Cross‚ a fair-skinned‚ red-haired South Asian studies major‚ who left India a few days before the deadly gang rape of a 23-year-old woman in New Delhi‚ said that helped others understand what she and her classmates went through. RELATED Loading WASHINGTON: Back from a study trip
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The Analysis of the Text “Up The Down Staircase” 1. The extract called «Up the down staircase» is written by Bel Kaufman‚ who is an American professor and writer. She is best known for this novel. The semi-autobiographical novel is about an idealistic young teacher. 2. The subject matter of this extract is school life. 3. The events happen in the middle of the 20th century‚ in the US Public School The plot is developed on the basis of the conflict between different sets of values.
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run down your spine giving everyone the chills. In the book “If I Grow Up” there are many spine chilling events which cause a lot of suspense. There are many scenes like the one mentioned where any character in the book could be killed at any moment due to gang violence. There are many key points which will be developed like a synopsis‚ an evaluation of the book‚ and an analysis of the main character. In the novel “If I grow up” there is a clear idea in which everything bases upon which are decisions
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In Ridley Scott’s Kingdom of Heaven the story’s protagonist‚ Balian‚ is the epitome of a true and perfect knight. Balian hasn’t always been a knight though. He used to be a simple blacksmith‚ but his life changes after his wife committed suicide after they lost their child. Then a knight named Godfrey came to him and told Balian that he was his illegitimate son and that started Balian’s journey to being a great knight just like his father. In order for Balian to be a true and perfect knight he has
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that explores the concept of love throughout several of his stories. In Ernest Hemingway’s “Up In Michigan”‚ the concept of love is explored through an innocent girl. Liz is in love with Jim‚ but he does not notice her often. When Jim finally gives Liz attention‚ it does not go as she plans. She gets raped. Critics argue that Liz is not truly in love‚ but it is clear that the feelings she exhibits is love. “Up In Michigan” is a story about an innocent girl who gets taken advantage of because of her
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