Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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Analysis and Separation of Organic Acids in White Wine Using High Performance Liquid Chromatography Atis‚ Arnelson Arwin G. and Gaitos‚ Gerald M. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City Performed: December 9‚ 2011 Submitted: December 15‚ 2011 RESULTS AND DISCUSSION solutions containing increasing amounts of the organic acid. These solutions were contained in flasks numbered 5‚ 6‚ 7‚ 8‚ and 9. (Refer to
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experiment is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served
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Chromatography of Food Dye Chem 180-DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring‚ M&M’s‚ Kool-Aid‚ and the dyes that came with the lab kit. Experiment and Observation: In this experiment a 50mL beaker was filled with luke warm water and a pinch of salt water was dissolved in it
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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Candy chromatography My experiment is called candy chromatography. This project is mainly about the colors that are mixed with other colors to make candy markers Koolaid and much more. I think that only the color of the candy is going to show up on the coffee paper. I think that threw the entire experiment the color in the candy is going to show up the entire time. I think this is going to happen because most of the colors are solid colors. The material and equipment used was a sample of candy
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9.1 Orbitals and Theories of Chemical Bonding 1. Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct? a) MO theory predicts that electrons are localized between pairs of atoms. b) In VB theory‚ bonding electrons are delocalized over the molecule. c) MO theory accurately describes bonding in O2 and NO‚ VB theory does not. d) VB theory can describe molecular bonding in excited states. e) MO theory is used to accurately predict
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The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
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