Experiment 3: Isolation of Chlorophyll from Spinach and Chromatography Performed February 14th and 16th By Kyle Herzog Organic Chemistry 344 Section 807 Spring 2012 Objective: The purpose of the experiment is to extract pigments from spinach leaves and separate them by column and thin layer chromatography‚ determining Rf values for the pigments. Equations: Rf= Distance travelled by a given compound/Distance travelled by a solvent solution 1. Crude- 4.25/5.5= 0.773 2. Carotene-
Premium
Simple Distillation‚ Gas Chromatography: Preparation of Synthetic Banana Oil Introduction This experiment gave us the opportunity to work with a variety of new procedures as well as practice procedures that are new to us within the past few weeks of labs. Within the context of the scenario‚ we find that a distilling company that markets a popular banana liqueur is having problems. The banana plantation that they use to create their banana extract was hit hard by a hurricane and their reserves
Premium Ester Distillation Acetic acid
Abstract Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it
Premium Color Chemical polarity Chromatography
1-hexanol D-4 #1‚2 C=O‚ -CH Cannot conclude Preparative Gas Chromatography was used to separate volatile compounds‚ which can then be identified. The injected sample becomes vaporized and was carried through the mobile column. It is usually carried by an inert gas. The gases were moved through the stationary column. The polarity of the components to the column would cause the separation of the components. Opposite to a TLC‚ if the stationary column was very polar‚ the more polar components will fall behind
Premium Chromatography Gas chromatography Chemistry
Alkaline Phosphatase was isolated using various techniques. BCIP spot test was performed after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each
Premium Protein Chemistry Acid
Lab #3 Separating Metal Cations by Paper Chromatography Introduction/Purpose: In chemistry‚ it is important to effectively analyze the components in a given mixture. In order to do so‚ chemists will analyze a mixture either qualitatively or quantitatively using different methods and equipment. Qualitative analyses are subjective‚ and only gather “non-numerical” data such as the color‚ appearance‚ and smell of the components. This data
Premium
Chemistry 266 Dominick Armstrong 10/10/2012 Acid-Base Titration Introduction Acid and bases are two important classes of compounds that react to form a salt and water. When mixing acids and bases a precise amount of the base must be added in order to reach the equivalence point. At this point‚ one reactant has been exactly consumed by the addition of the other reactant. When performing chemical reactions chemist use a process called titration to determine the equivalence point of the
Premium Titration Chemistry Acid
Is Higher Education Worth the Price? The question of whether or not higher education is actually “worth it” is an entirely subjective on. To objectively determine if higher education will give a high return on investment‚ one needs to first understand what they are hoping to gain out of the entire experience. Higher education does worth the price because it helps students to build their self-fulfillment‚ provide more monetary opportunities and reach their final goal . In the United States‚ for
Premium Higher education Academic degree Master's degree
CHAPTER 4 RESULTS AND DISCUSSION 4.1 Identification of camphor and menthol using gas chromatography – mass spectrometry (GC – MS) In this study‚ the presence of menthol and camphor were determined from 5 different locally produced medicinal topical ointment samples. Sample of Vick® VapoRub® and Hurix’s Massage Balm were purchased from local pharmacies‚ Bunga Cengkih Balm and Serai Balm samples were purchased from the grocery shop. Buah Pala Balm sample was purchased from the night market
Premium Gas chromatography Topical Menthol
Upon first examining the title of this poem‚ “The Layers‚” I predicted that the poem would be about a person’s “layers‚” such as their personality‚ and who they present themselves to be on the outside versus who they really are on the inside. After reading the poem‚ I found the title in the line “Live in the layers‚ not on the litter.” In context of the poem‚ I’d say this line and the title are using layers to represent different parts of our lives. Throughout the poem‚ the speaker is exploring and
Premium Poetry Stanza Linguistics