Cathedral What is your cathedral‚ meaning turning point or unforgettable moment that changed your life? In every people’s life there is something really important in order to think differently. In my life‚ the unforgettable moment is our university exam results clarification day. It was came from 2 years ago. It was my hardest year I was studying my dream university. Saying it honestly‚ I was not a diligent student as the same as the same as this year ; however‚ I can use advantages in correct
Premium Mario Super Mario Bros. Citrus
Transformation of E. coli with your unknown plasmid Learning Goals: Insert your uncut unknown plasmid into chemically competent DH-5 E.coli cells and use antibiotic resistance to confirm the success of the transformation. You should familiarize yourself with the various methods of transformation and the advantages/disadvantages of each type. You should also understand how heat shock transformation works and how chemically competent cells make this type of transformation possible. For this transformation
Premium Molecular biology DNA
Brittany Exam 3 Essay 9 Dec 2012 Transformations of the American West The three most important transformations of the American West were the completion of the railway system‚ economically‚ the diversity of the people‚ socially‚ and the conservation of land‚ politically. All of these transformations have made an unmistakable impact of the American West and what it is today. The largest economic transformation in American Western history was the building of the railroad systems. It was the
Premium American Old West Native Americans in the United States Cowboy
Running head: ORGANIZATIONAL TRANSFORMATION Q & A Organizational Transformation Q & A Jose Luis Sandoval Jr. OI/361 December 1‚ 2014 Michele Dougherty 1 ORGANIZATIONAL TRANSFORMATION Q & A 2 Organizational Transformation Q & A How incentives‚ training‚ and education are important to a company and also how important a strong leader is to a company. Jose will define and explained these topics. He will also compare these topics to his previous employment (Gill’s Auto Glass) and explain the positive
Premium Motivation Reward system Employment
Mike Wilcox Eng. 113 12-1250 Mr. Canipe 28 November 2007 Blindness is considered a disability. The person with a disability in Raymond Carver’s “Cathedral” is Bub. A person can be handicapped mentally. Bub is self-centered‚ and lives inside his own world. He is “blind” to the world around him and does not wish to open his mind to anything outside of his ignorant‚ pathetic‚ mundane life. Robert opens Bub’s mind‚ enabling Bub to see Robert as a person first‚ and not a blind man. Robert is
Premium Raymond Carver Fiction Narrative
Bacterial Transformation Lab Introduction: In this experiment we transformed a strain of E. Coli bacteria without antibiotic resistance with plasmid DNA. This plasmid produces a fluorescent green glow under black light due to the gfp(green fluorescent protein) as well as antibiotic resistance. E. Coli cells will be plated on an agar medium‚ some with and some without the antibiotic ampicillin. Only bacterial cells that contain the plasmid will survive the ampicillin and produce the green glow
Premium Bacteria DNA Escherichia coli
and in position C26 have been reported. 11 α hydroxylation is regarded as essential for anti-inflammatory action. Present study was promoted to extend the investigations to evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 α hydroxyprogesterone is the metabolite identified having 60
Premium Ribosomal RNA Bacteria Steroid
Who is the Real Blind Man? In the story Cathedral‚ by Raymond Carver‚ the narrator makes multiple statements as to how he feels about Robert‚ the blind man. The statements are made purely on what the narrator has seen in movies and what he has read. The narrator makes harsh judgments of the blind mind regarding his inability to see his surroundings and other people. However‚ the narrator fails to see things the same way that Robert does‚ more deeply. Throughout the story the narrator finds himself
Premium Raymond Carver Fiction Narrative
In Kafka’s The Metamorphosis‚ Gregor’s transformation into an insect is a supernatural agency that drives the plot‚ but it is ultimately just a way to show how his surrounding influence his life just as much as the transformation does‚ as the surroundings remind Gregor of his humanity and reveal the metamorphoses of the other characters. To start off‚ Gregor’s family very much depended on Gregor for their financial status. He worked hard as a travelling salesman‚ despite his hate for his boss
Premium The Metamorphosis Franz Kafka Vladimir Nabokov
While many short stories include the development of characters’ thoughts and a conclusion that ties loose ends together to help convey a theme‚ The Bath by Raymond Carver purposely excludes these elements to develop a theme. The Bath omits majority of the characters’ thoughts and feelings and does not have a conclusive ending‚ thus not communicating a clear understanding of the story to the reader and contributing to the theme of a lack of communication. Throughout the story‚ very little of the character’s
Premium Fiction Short story The Reader