Microbial Transformation of Steroids
HYDROXYLATION OF PROGESTERONE BY Bacillus circulans Strain Tex 01-S3c ISOLATED FROM OIL CONTAMINATED SOIL IN OMERGA REGION, MAHARASHTRA, INDIA
S. B. MALI & V.S. HAMDE
Dept. of Microbiology, Adarsh College, Omerga Dist. Osmanabad, 413606 malisiromerga@yahoo.com and Dept. of microbiology, Yogeshwari College, ambajogai, Dist. Beed. Maharashtra, India
Abstract
Hydroxylations are possibly the most widespread type of steroid bioconversion. Hydroxylations can be used to buildintermediates for further chemical synthesis to provide the steroid molecule with the adequate structure for therapeutic applications. Microorganisms able to hydroxylate steroids in positions C1 to C21 and in position C26 have been reported. 11 α hydroxylation is regarded as essential for anti-inflammatory action. Present study was promoted to extend the investigations to evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 α hydroxyprogesterone is the metabolite identified having 60 percent of yield. and Bacillus circulans Tex 01-S3c has been found most efficient strain.
Key words: -
Hydroxylation of Progesterone, oil contaminated, Bacillus circulans Tex 01-S3c,
INTRODUCTION
Application of molecular biology techniques and genetic engineering of microorganisms for their improvement as steroid transforming agents are the areas of drug development. Site selection oxygenation, particularly hydroxylation of exogenous steroids is frequent in bacteria genera Bacillus. Several microbial bioconversion of steroids and sterols have been reported, focusing mainly on steroid hydroxylations, ∆1-dehydrogenation and sterol side-chain cleavage. These biotransformations, mostly associated to chemical synthesis steps, have provided adequate tools for the large scale
S. B. MALI & V.S. HAMDE
Dept. of Microbiology, Adarsh College, Omerga Dist. Osmanabad, 413606 malisiromerga@yahoo.com and Dept. of microbiology, Yogeshwari College, ambajogai, Dist. Beed. Maharashtra, India
Abstract
Hydroxylations are possibly the most widespread type of steroid bioconversion. Hydroxylations can be used to buildintermediates for further chemical synthesis to provide the steroid molecule with the adequate structure for therapeutic applications. Microorganisms able to hydroxylate steroids in positions C1 to C21 and in position C26 have been reported. 11 α hydroxylation is regarded as essential for anti-inflammatory action. Present study was promoted to extend the investigations to evaluate potentiality of this local oil contaminated soil isolates for transformation of progesterone. Enrichment is carried out in the inorganic medium providing progesterone as an only carbon source. Metabolites are identified by GC/MS and isolates by 16 s rRNA. 11 α hydroxyprogesterone is the metabolite identified having 60 percent of yield. and Bacillus circulans Tex 01-S3c has been found most efficient strain.
Key words: -
Hydroxylation of Progesterone, oil contaminated, Bacillus circulans Tex 01-S3c,
INTRODUCTION
Application of molecular biology techniques and genetic engineering of microorganisms for their improvement as steroid transforming agents are the areas of drug development. Site selection oxygenation, particularly hydroxylation of exogenous steroids is frequent in bacteria genera Bacillus. Several microbial bioconversion of steroids and sterols have been reported, focusing mainly on steroid hydroxylations, ∆1-dehydrogenation and sterol side-chain cleavage. These biotransformations, mostly associated to chemical synthesis steps, have provided adequate tools for the large scale