3.4.2 Precautionary Measures Before And During The Study
3.4.3.2 Precautionary measures before and during the test procedures
The expiry dates of all the ELISA reagents provided were noted and the reagents were stored at 2 - 8°C when not in use. The ELISA strips contained in foil packs were allowed to warm to room temperature before opening to prevent condensation. Once opened, the micro-titration strips were stored at 2-8°C until use. All samples and reagents were kept at room temperature (20 - 25 °C) before and during the testing, but were returned to 2 – 8°C immediately after use.
For each sample, a new pipette tip was used and precautionary measures were taken never to touch the pipette tip to the bottom of the well. All the used materials were disposed off as bio-hazardous waste.
3.4.4 Data …show more content…
The response variable was thus binary, indicating whether the primate was positive for SFV or negative. The independent covariates comprised the baseline demographic and geographic variables that included study sites, primate ranging type, primate type, gender and age group. These laboratory results (presence or absence) and primate information were entered into a Microsoft Excel spreadsheet and later exported to International Business Machines Statistical Package for the Social Sciences version 21.0 (IBM SPSS v21.0). IBM SPSS v21.0 was then used to calculate the prevalence and perform a two-tailed Fisher’s exact test (for primate site) and chi-square test (for all other variables) for statistical significance. A 95% confidence intervals using the same software were calculated based on binomial "exact" calculations. The data analysed are represented in tabular and graphical …show more content…
The population for this research are those working and in close contact with primates at the various primate sites in Uganda.
3.5.2 Sample size, allocation and selection
The number of people working in close contact with primates in Uganda is unknown, but during the preliminary study, an estimated 300 people were found to work with primates at the five primates study sites (Personal communication with heads of study sites). Using a 5% margin of error, 95% confidence interval and 80% response rate, a sample size of 136 was calculated using a Raosoft® sample size calculator (http://www.raosoft.com).
The 136 participants for the questionnaire were selected using a proportional stratified two-stage, non-random sampling. First, the sample population was allocated to six different strata basing on the five primate site from which the primates were captured (Six, noted as “others”, to include people who have worked in any of the five primate sites, but not at the site during the study period. This form the majority of the study population).
Table 3.2: Sample allocation in the five study
The expiry dates of all the ELISA reagents provided were noted and the reagents were stored at 2 - 8°C when not in use. The ELISA strips contained in foil packs were allowed to warm to room temperature before opening to prevent condensation. Once opened, the micro-titration strips were stored at 2-8°C until use. All samples and reagents were kept at room temperature (20 - 25 °C) before and during the testing, but were returned to 2 – 8°C immediately after use.
For each sample, a new pipette tip was used and precautionary measures were taken never to touch the pipette tip to the bottom of the well. All the used materials were disposed off as bio-hazardous waste.
3.4.4 Data …show more content…
The response variable was thus binary, indicating whether the primate was positive for SFV or negative. The independent covariates comprised the baseline demographic and geographic variables that included study sites, primate ranging type, primate type, gender and age group. These laboratory results (presence or absence) and primate information were entered into a Microsoft Excel spreadsheet and later exported to International Business Machines Statistical Package for the Social Sciences version 21.0 (IBM SPSS v21.0). IBM SPSS v21.0 was then used to calculate the prevalence and perform a two-tailed Fisher’s exact test (for primate site) and chi-square test (for all other variables) for statistical significance. A 95% confidence intervals using the same software were calculated based on binomial "exact" calculations. The data analysed are represented in tabular and graphical …show more content…
The population for this research are those working and in close contact with primates at the various primate sites in Uganda.
3.5.2 Sample size, allocation and selection
The number of people working in close contact with primates in Uganda is unknown, but during the preliminary study, an estimated 300 people were found to work with primates at the five primates study sites (Personal communication with heads of study sites). Using a 5% margin of error, 95% confidence interval and 80% response rate, a sample size of 136 was calculated using a Raosoft® sample size calculator (http://www.raosoft.com).
The 136 participants for the questionnaire were selected using a proportional stratified two-stage, non-random sampling. First, the sample population was allocated to six different strata basing on the five primate site from which the primates were captured (Six, noted as “others”, to include people who have worked in any of the five primate sites, but not at the site during the study period. This form the majority of the study population).
Table 3.2: Sample allocation in the five study