Preview

Amylase And Starch Lab Report

Satisfactory Essays
Open Document
Open Document
393 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Amylase And Starch Lab Report
What amount of amylase is the best to produce a better reaction with starch?

A 1% solution of amylase, made from 0.15g of amylase added to 15ml of water, a 2% solution of amylase made of 0.3g of amylase added to 15ml of water, and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created, and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test tube, 4ml of the 2% solution was added to the second test tube, 4ml of the of the 0.5% solution was added to the third test tube and 4ml of water was added to the fourth test tube as a control. As soon as the amylase was added, the stop watch started starts to time how long it takes for the blue to fade away (not completely colourless). If the reaction didn't occur within three minutes, the stop watch was stopped. The rate of reaction was measured using this formula: 1/time (sec)
…show more content…
The original experiment asked for only a 1% amylase made from 0.1g to 10ml of water. A larger range was used to get a wider knowledge on how amylase affects starch. Multiple test tubes were used for each solution to create multiple tests to get a more accurate result for

You May Also Find These Documents Helpful

  • Good Essays

    Biol 111 lab report water

    • 656 Words
    • 3 Pages

    By having a control in this experiment, we can see the changes with the presence of amylase. Lugol’s regent (I2Kl) changes color in the presence of starch, which is the control group that we have setup that demonstrates what would happen naturally, with starch and Lugol’s regent (I2Kl). The control group will have a distinct comparison available for us. As amylase, an enzyme that catalyzes the breakdown of the starch into glucose, which will have no reaction with Lugol’s regent (I2Kl) as well as no color change.…

    • 656 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Lab 2

    • 559 Words
    • 3 Pages

    In the test for starches, water again will serve a the negative control for the experiment becasue it is inorganic. it appears obvious the 1% starch solution will test positve for starches becasue it is starch. The onion juice should carry starch due to the fact that starch is stored energy for plants and onions are very bulbous, while the sucrose solution again will not test positive because sucrose is only a part of starch.…

    • 559 Words
    • 3 Pages
    Satisfactory Essays
  • Satisfactory Essays

    Enzymes Lab Report

    • 371 Words
    • 2 Pages

    Explain in detail the procedure that you followed (including amount of substrate, enzyme etc, and the whole procedure including incubation times) (3 Points)…

    • 371 Words
    • 2 Pages
    Satisfactory Essays
  • Powerful Essays

    Crystal Violet Formal Lab

    • 2472 Words
    • 10 Pages

    Introduction: Kinetics, which is the study of how fast a reaction takes place or in other words the rate of a reaction, is the main ideology in this experiment. Reaction rates can be measured in a number of ways: by monitoring the amount of product formed, by measuring the loss in mass of reactants, for reactions involving gaseous products measuring the volume of gas produced, by electrolytic conductivity, pH measurement or for colored reactants or products measuring the transmittance by the use of a colorimeter. In this experiment the last method of measurement is used which is colorimetry.…

    • 2472 Words
    • 10 Pages
    Powerful Essays
  • Good Essays

    Exercise 8 Physioex 8.0

    • 858 Words
    • 4 Pages

    a. maximum of amylase is at pH 7.0 (tubes 2 & 5, brownish red) and pH 9.0 showed little activity (tubes 6 & 7, green)…

    • 858 Words
    • 4 Pages
    Good Essays
  • Good Essays

    Amylase Lab

    • 832 Words
    • 4 Pages

    In table 1 are the results for the bacterial amylase, what is depicted is the minutes passed and the number corresponding to the color most similar to the color chart provided by the lab manual. The results showed that the shade of yellow was lightest at 55 degrees. In table 2 the same information is recorded, showing once again the most activity at 55 degrees celsius. Picture 1 was taken by the group, showed the results of both the bacterial and fungal amylase and picture 2 shows the color chart referenced for the results of the charts. Graph 1 (bacterial) and graph 2 (fungal) represents the enzyme activity vs. change in temperature, they had similar results although the bacterial amylase had a higher activity…

    • 832 Words
    • 4 Pages
    Good Essays
  • Better Essays

    amylase lab report

    • 1766 Words
    • 8 Pages

    Cited: Campbell, Neil A., Jane B. Reese, Martha R. Taylor, Eric J. Simon. Biology 105 Taken From; Biology: Concepts & Connections (Fifth Edition) Benjamin Cumming, San Francisco, CA, 2013.…

    • 1766 Words
    • 8 Pages
    Better Essays
  • Good Essays

    Enzyme Lab Write Up

    • 724 Words
    • 3 Pages

    We then repeated this in two more test tubes. We put one of the test tubes in ice (0 C) we put another in the water bath (42 C) then we put one in boiling water (100 C). We repeated the same thing for the boiled amylase. We then checked at five minutes to see if the starch had been digested by the amylase. We did this by putting a little of each of the six samples into a spotting tile. We then added iodine solution to this to see if starch was present or not. I could tell if starch was present or not because if the solution went orange it meant that there was no starch present, so all the starch had been digested, if the solution went blue/black it meant that there was starch present, so the starch had not been fully digested. We recorded the results in a table. We then waited another two minutes so that all together the starch solution had been in there seven minutes. We did exactly the same thing as we did at five minutes we also recorded these results in a…

    • 724 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Starch Lab

    • 532 Words
    • 3 Pages

    Introduction: In this lab i used glucose and starch to show if the cell membrane is permeable, impermeable, or selectively permeable to these substances. Dialysis tubing is a plastic like substance that has small pores in it. These pores allow certain chemicals to flow into and out of the cells. The size of the molecule determines what goes through the tubing. On of the materials that we will be using is glucose. glucose is a simple sugar that easily dissolves in water. To test for the presence of glucose we will use glucose test strips. The strip will change from an aqua blue color to a brown color if there is glucose in the solution. Starch is a complex molecule that forms from a suspension in the water. To test for the presence of starch I will use Lugol's iodin. When it comes in contact with the water and turns black or blue thats how you know that starch is present.…

    • 532 Words
    • 3 Pages
    Good Essays
  • Powerful Essays

    Lab Report

    • 2214 Words
    • 9 Pages

    Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1, 4, 7, 10, and 14) on the function of amylase by using starch and iodine.…

    • 2214 Words
    • 9 Pages
    Powerful Essays
  • Good Essays

    Enzyme Lab Report

    • 1528 Words
    • 5 Pages

    experiment is to determine what changes in pH, temperature, and enzyme concentration have on the rate…

    • 1528 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Amylases are starch-degrading enzymes that catalyze the hydrolysis of internal glycosidic bonds in polysaccharides (Sivaramakrishnan et al., 2006). To be effective, enzymes require specific environmental and chemical conditions. For example, the slightest variation in pH levels can significantly…

    • 1625 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Kinetic Reaction Lab

    • 719 Words
    • 3 Pages

    The data was then plotted as concentration vs. time (Figure 1), ln concentration vs. time (Figure 2), and 1/concentration vs. time (Figure 3). Since a linear graph was assembled using the data set of ln concentration vs. time, the blue food coloring solution was determined to be first order in this reaction. The slope of the linear graph was determined to be -0.4581, and since its absolute value is equal to the observed rate constant, kobs, the observed rate constant equaled 0.4581.…

    • 719 Words
    • 3 Pages
    Good Essays
  • Better Essays

    The purpose of this experiment was to determine (1) the reaction rate of an amylase enzyme in starch and (2) the environmental factors that can affect the enzymatic activity. The hypothesis, in relation to the enzymatic activity by variables such as the substrate concentrations, temperature, PH and chemical interactions on the rate of reaction, stated the following scenarios: (1) If the substrate concentration is increased, then the enzymatic rate will increase (2) If the temperature is increased, then the enzymatic rate will also increase (3) If the PH level is increased, then the enzymatic rate will decrease.…

    • 2678 Words
    • 11 Pages
    Better Essays
  • Powerful Essays

    This experiment focuses on how the change of temperature affects the rate of reaction of amylase. In the experiment there were four different environments that each contained 2 test tubes. Each test tube consisted of the same concentration and amount of starch and amylase. After having each test tube placed in these environments for several minutes a droplets of each mixture was placed onto each slot which contained liquid solution of iodine. Each environment contained two different test tubes, one test tube was starch and the other was amylase. A droplet was placed in ten to thirty second intervals to see the different states of starch break up. When starch is present with iodine, the solution is a dark and transparent purple color, when there is less starch present the intensity of the color purple decreases. The the objective of this experiment was whether the change in temperature and the speed of reactivity of amylase is directly proportional or not. The hypothesis was with the increase in temperature there would be an increase in the speed of reactivity of amylase with starch. Results showed that with the increase in temperature there is an increase in the speed but after 37ºC the rate of reaction decreased. This proved that there is an optimum temperature point for the rate of reaction for amylase which was 37ºC.…

    • 1387 Words
    • 6 Pages
    Powerful Essays