A 1% solution of amylase, made from 0.15g of amylase added to 15ml of water, a 2% solution of amylase made of 0.3g of amylase added to 15ml of water, and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created, and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test tube, 4ml of the 2% solution was added to the second test tube, 4ml of the of the 0.5% solution was added to the third test tube and 4ml of water was added to the fourth test tube as a control. As soon as the amylase was added, the stop watch started starts to time how long it takes for the blue to fade away (not completely colourless). If the reaction didn't occur within three minutes, the stop watch was stopped. The rate of reaction was measured using this formula: 1/time (sec) …show more content…
The original experiment asked for only a 1% amylase made from 0.1g to 10ml of water. A larger range was used to get a wider knowledge on how amylase affects starch. Multiple test tubes were used for each solution to create multiple tests to get a more accurate result for