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Anaerobic Respiration Lab Report

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Anaerobic Respiration Lab Report
Investigating how different temperatures will affect the rate of anaerobic respiration in yeast.

Research and Rationale:
My investigation consists of researching and carrying out experiments in order to obtain results on how the rate of respiration is affected by temperature. In the experiment I will use one sugar but will be changing the temperatures. In my investigation I will carry out an experiment where one sugar is used to test how changes in temperature (10⁰C - 60⁰C) affect the rate of anaerobic respiration in yeast. I will be carrying out a preliminary experiment using four different sugars. Each of these sugars will be tested at 10⁰C, 20⁰C, 30⁰C, 40⁰C, 50⁰C and 60⁰C to see which sugar has the most appropriate rate of reaction and results in noticeable and recordable differences. This will be the sugar that I will use to carry out my experiment. The four different sugars that will be tested in
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Because the monosaccharide is not stable it needs to be broken down into two 3-carbon molecules, and this is pyruvate. The process that splits the monosaccharide into two molecules of pyruvate called glycolysis. Glycolysis is an energy dependent process; however it can not get its energy directly from sugars such as glucose so an alternative is needed. During respiration energy is released, that energy is then converted into ATP. ATP is made from inorganic phosphate and ADP. The energy released from respiration is then stored in the bond (phosphate bond) in ATP. This supplies the energy for glycolysis to take place to produce pyruvate. So when respiration takes place in yeast carbon dioxide is removed from pyruvate this reaction is catalysed by the enzyme pyruvate decarboxylase. The pyruvate molecule from the monosaccharide is converted into an alcohol, usually ethanol and carbon dioxide is

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