anchorage dependent cell culture
Anchorage Dependent Animal Cell Culture
Animal Cell Culture: Its origin and importance
Prior to discussing about the anchorage dependence of animal cell cultures, let us first briefly discuss animal cell culture in general and its importance. Animal cell culture has a history of over 100 years when the first attempts to grow cells were made by Ross Harrison who was able to show the development of nerve fibres from frog embryo tissue cultured in a blood clot. Physiologist Alexis Carrel was able to keep fragments of chick embryo heart alive and beating for three months in vitro. With the help of subsequent research and technology development, animal cell cultures have been used for in vitro experimental studies and also for production of numerous useful products such as interferon, erythropoietin, tissue plasminogen activator and other biopharmaceuticals. More recently, interest has also developed in the application of stem cell technology for cell based therapy. Animal cell cultures have also been used as vehicles to grow viruses for development of vaccines and to study viral infections in mammals.
Thus, animal cell culture technology has found wide application which makes it indispensable to understand the essential features of animal cell growth in culture. Animal cells, unlike microbial or plant cells, are very fastidious and complex in their nutritional requirements and require special incubators wherein the levels of CO2, O2 and humidity are maintained similar to those prevailing in the source animal’s body. Animal cells can grow as adherent cells or suspension cultures. Some of the important features of animal cell cultures are as follows:
Features of animal cell growth in culture
Animal cells, depending on the tissue they have been isolated from, can grow even in the best nutritive media to only limited generations.
They divide and fill the surface of the container they are growing in and then stop growing. The cells comprising tissues and
Animal Cell Culture: Its origin and importance
Prior to discussing about the anchorage dependence of animal cell cultures, let us first briefly discuss animal cell culture in general and its importance. Animal cell culture has a history of over 100 years when the first attempts to grow cells were made by Ross Harrison who was able to show the development of nerve fibres from frog embryo tissue cultured in a blood clot. Physiologist Alexis Carrel was able to keep fragments of chick embryo heart alive and beating for three months in vitro. With the help of subsequent research and technology development, animal cell cultures have been used for in vitro experimental studies and also for production of numerous useful products such as interferon, erythropoietin, tissue plasminogen activator and other biopharmaceuticals. More recently, interest has also developed in the application of stem cell technology for cell based therapy. Animal cell cultures have also been used as vehicles to grow viruses for development of vaccines and to study viral infections in mammals.
Thus, animal cell culture technology has found wide application which makes it indispensable to understand the essential features of animal cell growth in culture. Animal cells, unlike microbial or plant cells, are very fastidious and complex in their nutritional requirements and require special incubators wherein the levels of CO2, O2 and humidity are maintained similar to those prevailing in the source animal’s body. Animal cells can grow as adherent cells or suspension cultures. Some of the important features of animal cell cultures are as follows:
Features of animal cell growth in culture
Animal cells, depending on the tissue they have been isolated from, can grow even in the best nutritive media to only limited generations.
They divide and fill the surface of the container they are growing in and then stop growing. The cells comprising tissues and