Pericarp Fruit via Choriollantoic Membrane Assay: A Potential
Agent against Tumour Vascularisation
R. J. I. Tambole, M. J. K. Peteros, J. J. Alegado
Bayugan National Comprehensive High School
ABSTRACT
Cancer has been a leading cause of death worldwide and will still be the same on the future if necessary actions will not be done. A lot of people can be affected by this disease yet not all of them have the access of expensive and artificial treatments. The researchers are urged to discover a natural and effective for stopping tumour vascularisation. In this study, the pericarp of the Calabash (Crescentia Cujete) is utilized as an effective treatment for stopping tumour vascularisation.The obtained 457g of Calabash Fruit pericarp was soaked by solvent extraction using 1.5L 95%-Hexane and 1.5L 95%-Ethanol for 24 hours each. The extract was filtered and evaporated using rotary evaporator for 3 hours and further dried for 24 hours. The residual crude extract was poured in a glass container. The extracts were tested through Chorioallontoic Membrane Assay (CAM).The CAM Assay gave the following results. The mesh indices of the different concentration (25%, 50%, 100%) of the extracts, and the positive and negative control were the following: 285.663, 280.534, 166.008, 176.162, and 244.049.The result of the Chorioallontoic Membrane Assay showed that the extract showed no significant difference to the positive control so it is showed that the extract also has anti-angiogenic effect to the chick embryo. The anti-angiogenic property only shows when 100% concentration (dose-dependent) of the extract is injected to the egg. The researcher concluded that the Calabash Fruit Pericarp has anti-angiogenic effect thus having the potential of halting tumor vascularisation.
INTRODUCTION
Cancer is a malignant tissue (tumour) that generated from the result of the continuous proliferation of