Microbes such as bacteria are continually progressing that enables them to easily adapt a new environment. Their presence causes health problems to human and animals because they are multiplying rapidly and they are focusing on the regions that …show more content…
in 2010, there group focused on the antibacterial effect (in vitro) of Moringa oleifera and Annona muricata against the gram positive and negative bacteria. The solvents that were used in the experiment were ethanol and water to extract the seeds of moringa (Moringa oleifera) and the pods of soursop (Annona muricata) with a ratio of 1:50 and 1:10 and a concentration of 50, 100, 150 and 200 μL. Vibrio cholerae, Salmonella enteritidis, Staphylococcus aureus, and Escherichia coli were used as the test organisms in the experiment. Escherichia coli was isolated from a local lake, river, and agriculture livestock which was the Pacific white shrimp (Litopenaeus vannamei) and Tilapia (Oreochromis