Ap Biology Lab Report

Diffusion and Osmosis Through Dialysis tubing

We did this experiment to test the diffusion of different substances through dialysis tubing. We used what we knew about diffusion to make predictions on what we thought the mass of the dialysis tubing to be after submerging them for 30 mins and we knew that diffuse occurs from highest concentration to lowest concentration.

Since the dialysis tubings are filled with different substances than what they are being put into then they should all gain or lose mass. If the dialysis tubing is submerged in different substance than what is in the tubing then some of the dialysis tubings will lose mass and some will gain mass.

To begin this experiment we filled 5 dialysis tubings with one of the five substances: water, egg white, NaCl, glucose, or sucrose. The equipment used was: 5 dialysis tubing, a scale, bekers, water, egg white, sucrose, glucose, NaCl, and 5 cups. We put the five dialysis tubing filled with one of the five substance into a cup filled with one of the substance: water, egg white, NaCl, glucose, or sucrose. Then we let them sit in there for 30 mins then took them out of the cups and remassed them.

Our results were:

Original Mass of dialysis tubing Water Sucrose Glucose

NaCl Egg White

9g

9.8g

10.4g

9.8g

9.4g

Our substance combinations were:

water in water-water in the dialysis tubing submerged in a cup of water

sucrose in egg white-sucrose in the dialysis tubing submerged in a cup of egg white

glucose in NaCl-glucose in the dialysis tubing submerged in a cup of NaCl

NaCl in sucrose-NaCl in the dialysis tubing submerged in a cup of sucrose

egg white in glucose-egg white in the dialysis tubing submerged in a cup of glucose

Post Submerge Water Sucrose Glucose NaCl Egg White

9g

11.7g

11.8g

8g

8.7g

The dialysis tubing did let some of the different