Aspirin Synthesis Lab Report

High Performance Liquid Chromatography Analysis of Aspirin
Problem:
Was aspirin (acetylsalicylic acid) successfully synthesized?
Are there impurities or by-products present in the synthesized aspirin?
How pure is the synthesized aspirin?
Introduction:
In the last experiment, aspirin was synthesized followed by characterization of the product using several different techniques. Melting point was a test that provided information about the identity and purity of the aspirin product. The iron(III)chloride test was used to detect the presence of a phenol group, with a positive result indicating the reactant salicylic acid was leftover as an impurity. Thin layer chromatography (TLC) was also used to determine if the white powder synthesized
You will need to have peak heights and retention times recorded for three injections of each of the following: aspirin standards 1-4, salicylic acid, and synthesized aspirin sample.

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High Performance Liquid Chromatography Analysis of Aspirin
Name ____________________

Date__________________

Partner Name__________________

Section ________________

Results and Analysis:
Part I: Data
Preparation of Solutions:
Mass (mg)

Concentration in flask
(mg/mL)

Aspirin Std 1
Aspirin Std 2
Aspirin Std 3
Aspirin Std 4
Salicylic Acid
Aspirin Sample
HPLC Analysis: Aspirin Standards and Salicylic Acid
Sample
Retention Time
Aspirin Std 1 (inj.1)
Aspirin Std 1 (inj.2)
Aspirin Std 1 (inj.3)
Aspirin Std 1 (average)
Aspirin Std 2 (inj.1)
Aspirin Std 2 (inj.2)
Aspirin Std 2 (inj.3)
Aspirin Std 2 (average)
Aspirin Std 3 (inj.1)
Aspirin Std 3 (inj.2)
Aspirin Std 3 (inj.3)
Aspirin Std 3 (average)
Aspirin Std 4 (inj.1)
Aspirin Std 4 (inj.2)
Aspirin Std 4 (inj.3)
Aspirin Std 4 (average)
Salicylic Acid (inj. 1)
Salicylic Acid (inj. 2)
Salicylic Acid (inj. 3)
Salicylic Acid (ave.)

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Peak Height

HPLC Analysis: Synthesized aspirin sample
Record all major peaks in the chromatogram. Depending on the purity of the aspirin