Preview

Bacterial Unknown Report: 'Alyse Rose'

Satisfactory Essays
Open Document
Open Document
334 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Bacterial Unknown Report: 'Alyse Rose'
Alyse Rose
Microbiology Lab
Bacterial Unknown
March 25, 2013

Bacterial Unknown Report

Each student was given an unknown bacteria to figure out. I was given the unknown bacteria S38. Everybody is supposed to do all sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours.

1. GRAM STAIN
The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet, grams iodine, acetone-alcohol, and grams safranin. Under the microscope it was a pink color, which means Gram Negative. Also, the shape was a rod.

2. KLIGER’S IRON
Continue Reading
View Writing Issues

You May Also Find These Documents Helpful

  • Satisfactory Essays

    Lab Report On Unknown 32
    • 591 Words
    • 3 Pages

    Lab Report On Unknown 32

    The first test conducted on unknown bacteria 32 was the Gram stain. From this stain, unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria, Gram-positive rods and Gram-positive spirillium. Next, the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green, steam, and safranin it was found that unknown bacteria 32 did not contain endospores.…

    • 591 Words
    • 3 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Indole Test Lab Report
    • 1279 Words
    • 6 Pages

    Indole Test Lab Report

    My partner and I were given unknown number 3 in the laboratory. After performing various tests over the course of a few weeks on our unknown, we came to the conclusion that our unknown organism is Klebsiella pneumoniae (K. pneumoniae). K. pneumoniae is a gram negative bacillus shaped microorganism. We observed that K. pneumoniae is a nonmotile organism. We performed multiple tests on our unknown culture, therefore we are very confident that it is correctly identified.…

    • 1279 Words
    • 6 Pages
    Good Essays
    Read More
  • Better Essays

    Anatomy & Physiology Chp 22 Quiz
    • 1308 Words
    • 6 Pages

    Anatomy & Physiology Chp 22 Quiz

    Procedure: There are four parts to this experiment. The first step is to set up an incubation site. The incubation site needs to be an area that is out of the way, can maintain a constant temperature and is free from draught. Refer to the course information tab on the class website for instructions on how to build an incubator. The microbes in this experiment have different temperature requirements: one type is best grown at room temperature while the other requires a temperature similar to that of our physical body (between 35 and 37 degrees Celsius).…

    • 1308 Words
    • 6 Pages
    Better Essays
    Read More
  • Powerful Essays

    Unknown Bacteria Lab Assignment
    • 1738 Words
    • 7 Pages

    Unknown Bacteria Lab Assignment

    The purpose of the unknown bacteria lab assignment was to select an unknown bacteria culture and, through a series of metabolic tests, identify which bacteria genus resided in the pure culture received. A nutrient broth inoculated with bacterial culture (numbered 45, henceforth referenced as U45) was selected and a streak plate was made to isolate a pure culture for use throughout the assignment.…

    • 1738 Words
    • 7 Pages
    Powerful Essays
    Read More
  • Better Essays

    Microbiology Unknowns Lab Report
    • 1370 Words
    • 6 Pages

    Microbiology Unknowns Lab Report

    By doing these tests we will be able to see how the unknown bacteria reacts to certain conditions. If our results of these lab experiments are conclusive then we should be able to determine what our unknown bacteria is.…

    • 1370 Words
    • 6 Pages
    Better Essays
    Read More
  • Good Essays

    Unknown Lab Report
    • 673 Words
    • 3 Pages

    Unknown Lab Report

    I think we correctly identified our unknown as P. aeruginosa because we performed several different tests, eleven of which helped us identify our organism on the Gram negative chart that was in our lab book. When it came down to narrowing the organisms down to ours, I looked at the glucose test first because all of the other Gram negative organisms were positive for glucose except for two. Since our organism tested negative for glucose it helped narrow it down to only those two organisms, Alcaligenes faecalis and Pseudomonas aeruginosa. The other tests that we performed on our unknown were not really helpful when it came to determining if it was Alcaligenes faecalis or Pseudomonas aeruginosa because they both had all the same characteristics when it came to the lactose, mannitol, motility, indole, citrate, H2S, urease, methyl red and VP tests. Our tests for lactose, mannitol, indole, urease, methyl…

    • 673 Words
    • 3 Pages
    Good Essays
    Read More
  • Better Essays

    Unknown Lab Report
    • 2145 Words
    • 9 Pages

    Unknown Lab Report

    The tests performed on the unknown bacteria cultures were all used to determine the identity of the bacteria. Each of the tests performed provided some key information about the bacteria in question and how it functions. Not all of the tests were performed on every culture, however, as some of the tests were used only for gram (+) or gram (–) bacteria, while others were even more specific and used only for cocci bacteria. The tests performed and what constitutes a positive and negative test are as follows.…

    • 2145 Words
    • 9 Pages
    Better Essays
    Read More
  • Good Essays

    Unknown Lab Report
    • 1149 Words
    • 5 Pages

    Unknown Lab Report

    To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis, Staphylococcus saprophyticus, Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Salmonella [I assume typhimurium], or Shigella [either flexneri or sonnei, we used both in our lab during the semester].…

    • 1149 Words
    • 5 Pages
    Good Essays
    Read More
  • Good Essays

    Unknown Microorganism
    • 1335 Words
    • 6 Pages

    Unknown Microorganism

    The streak for isolation was chosen to see the formation of the different colonies and to see if the bacteria would cause growth on the plate. After observing the plate, it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative, other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete, it showed a yellow butt, yellow slant and gas production. This determined that the unknown ferments glucose and one or both of the other sugars. The gas was produced by fermentation. The color change in the agar brought the unknown down to two options of unknowns, Escherichia coli or Hafnia alvei. The SIM medium test was chosen next to see sulfur reduction, indole production from tryptophan…

    • 1335 Words
    • 6 Pages
    Good Essays
    Read More
  • Good Essays

    Unknown Bacteria Report
    • 586 Words
    • 3 Pages

    Unknown Bacteria Report

    An unknown bacterium experiment, this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al., 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by means of the Embden-Meyerhof pathway, they produce plasmid-encoded proteins and that some of them perform mixed acid fermentation, some use enzyme systems like formate dehydrogenase and pyruvate formate-lyase (PFL) The first thing that had to be done was the streak plating to isolate the bacterium, then the next lab multiple tests were ran to help identify which bacterium was in the sample. One of the most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium.…

    • 586 Words
    • 3 Pages
    Good Essays
    Read More
  • Good Essays

    Requirement for Unknown Report
    • 629 Words
    • 3 Pages

    Requirement for Unknown Report

    You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism, described their colonies on a TSA plate, and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells, colonies, growth temperature preferences, etc. in order to identify it. You will provide a report (no more than 4 single-spaced pages including the dichotomous key tree and table) on your identification that includes the following:…

    • 629 Words
    • 3 Pages
    Good Essays
    Read More
  • Satisfactory Essays

    Unknown Paper
    • 578 Words
    • 3 Pages

    Unknown Paper

    The unknown project was a very good realization of me and my partner going out by our selves. The very first day of the Unknown project I was acquainted with Denise who was very friendly and was very nice as far as assisting me in the project. The first day we were introduced too various different forms of the unknown such as broth, Blood agar plate, MSA plate, MAC plate, and the EMB plate. Before that we had done the 3 phase isolate which we had a possible of 10 points of achieving. In the isolation plate we had too take the sample of our unknown, which was the letter G. After we had done the 3-phase isolation plate we inoculated half of the plate, which was the S/D media. The Plate’s that we had provided too us were the BAP, MSA, MAC, and EMB. When we were successfully inoculated the S/D media the plates were put in the Incubator at approximated 37 degrees Celsius. The second day we had came into the laboratory we had too read the Nutrient Agar plate that was the one that we had too the 3 phase isolation plate. My results were somewhat correct but I had a lot of backtracking. After that we had too read the S/D Media, which we had, too diagram and describe each of the plates that we had put in the incubator. Once they had come out of the Incubator the results that I had achieved was the MSA plate was yellow, stinky, I did have growth, there were a positive match for small colonies, and was shiny. The second one that I had achieved was on the MAC plate. The color on this specific plate was red, it had smelled rotten, there was definitely some growth on the plate the texture was shiny and was not raised. Out of these plates I had somewhat of a indication of what the specimen was since after the gram stain we had done in lab we found out that on the MSA plate we had achieved Gram positive…

    • 578 Words
    • 3 Pages
    Satisfactory Essays
    Read More
  • Good Essays

    Unknown Bacteria
    • 1076 Words
    • 5 Pages

    Unknown Bacteria

    Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons, such as knowing the causative agent of disease, knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study. This study allows the student to exercise their ability to use and understand sterile techniques, transmission, and prevention of microorganisms.…

    • 1076 Words
    • 5 Pages
    Good Essays
    Read More
  • Good Essays

    Archetype, Oxidase Test
    • 551 Words
    • 3 Pages

    Archetype, Oxidase Test

    The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain, my unknown was the color pink. This revealed my unknown as being gram negative. I also used the microscope to identify the morphology of the organism. I recognized the unknown to be staphylobacillus. Staphylobacillus means rod shaped cells in grape-like clusters. After establishing the morphology and gram stain I had six options for what my unknown was.…

    • 551 Words
    • 3 Pages
    Good Essays
    Read More
  • Better Essays

    Gram Staining
    • 1318 Words
    • 6 Pages

    Gram Staining

    The gram staining procedure consists of fixing a colony of bacteria onto a slide and then flooding the colony with various chemicals. First, crystal violet dye was dropped onto the bacterial cells, staining gram-positive cells purple. Iodine was added to fix the violet dye into place and then ethanol was used to wash the dye off the unstained cells. Finally, a red dye called safranin was used to stain any gram-negative cells present so that they were visible. Once the gram stain procedure was complete, the gram-positive bacteria appeared purple under a microscope while gram-negative cells appear pink or red. The purpose of this lab was to differentiate bacteria based on the cell wall and to differentiate whether the bacteria was gram-positive or gram-negative. Gram-negative bacteria do not retain the violet dye and are colored red or pink. “Compared with Gram-positive bacteria, Gram-negative bacteria are more resistant against antibodies because of their impenetrable cell wall. These bacteria have a wide variety of applications ranging from medical treatment to industrial use and Swiss cheese production” (2).…

    • 1318 Words
    • 6 Pages
    Better Essays
    Read More

Related Topics