Beetroot
Beetr-------------------------------------------------
The Effect of Temperature on Membrane Permeability in Beetroot Cells
Stage 2 Biology 2013
Results Figure 1 Temperature | Absorbance at 530nm | 30°C | 0.407nm | 40°C | 0.457nm | 50°C | 0.518nm | 60°C | 0.622nm | 70°C | 0.672nm | 80°C | 0.859nm |
The table above gives an example of how a higher temperature has a greater effect on denaturing a membrane and hence the release of more pigment from the beetroot membrane. To obtain these results, first, beetroot samples were cut from a raw beetroot plant using a cork borer. From this, 30 discs 3mm thick and of uniform size were cut from the beetroot cylinders. After this process the beetroot discs were then washed in a beaker half filled with distilled water, this was done by agitating the water for three minutes and then draining the beaker through a strainer. This process was repeated 3 times until the water after the washing process appeared clear. After the beetroot discs were cleaned, 6 skewers were then impaled with 5 beetroot discs each, the discs were spread evenly apart down the bottom end of the skewer. 6 test tubes were filled with distilled water and then sat in a beaker on top of a water bath. Once each test tube had reached its desired temperature (30°C, 40°C, 50°C, 60°C, 70°C, or 80°C), a beetroot stack was then immersed into a test tube for exactly one minute, the test tube, with the beetroot stack still in it, was then left in a water bath at room temperature for 30 minutes. This process was repeated for all remaining test tubes. After this, results were recorded in the spectrophotometer.
Relationship between Heat and Pigment Release Figure 2
(nm)
(nm)
(°C)
(°C)
Both Figure 1 and Figure 2 display how temperature affects the
The Effect of Temperature on Membrane Permeability in Beetroot Cells
Stage 2 Biology 2013
Results Figure 1 Temperature | Absorbance at 530nm | 30°C | 0.407nm | 40°C | 0.457nm | 50°C | 0.518nm | 60°C | 0.622nm | 70°C | 0.672nm | 80°C | 0.859nm |
The table above gives an example of how a higher temperature has a greater effect on denaturing a membrane and hence the release of more pigment from the beetroot membrane. To obtain these results, first, beetroot samples were cut from a raw beetroot plant using a cork borer. From this, 30 discs 3mm thick and of uniform size were cut from the beetroot cylinders. After this process the beetroot discs were then washed in a beaker half filled with distilled water, this was done by agitating the water for three minutes and then draining the beaker through a strainer. This process was repeated 3 times until the water after the washing process appeared clear. After the beetroot discs were cleaned, 6 skewers were then impaled with 5 beetroot discs each, the discs were spread evenly apart down the bottom end of the skewer. 6 test tubes were filled with distilled water and then sat in a beaker on top of a water bath. Once each test tube had reached its desired temperature (30°C, 40°C, 50°C, 60°C, 70°C, or 80°C), a beetroot stack was then immersed into a test tube for exactly one minute, the test tube, with the beetroot stack still in it, was then left in a water bath at room temperature for 30 minutes. This process was repeated for all remaining test tubes. After this, results were recorded in the spectrophotometer.
Relationship between Heat and Pigment Release Figure 2
(nm)
(nm)
(°C)
(°C)
Both Figure 1 and Figure 2 display how temperature affects the