Biochemical Analysis of Blood
Abstract
Introduction
After blood is collected from a patient it is analysed to check the fragments present and the fluid which will either be plasma or serum. After plasma is separated from blood it recollects the fibrinogen which in turn helps in clotting however serum is the portion of blood which remains after the fibrinogen is removed. Serum contains approximately 6-8% of the proteins which make up blood however plasma creates up to 55% of blood. Serum contains different proteins which include albumin and globulins however plasma contains clotting factors, water and serum. In order for us to see the difference between plasma and serum we have to analyse the blood. If after removal, the blood does not clot then we know that the fluid present is a serum. If the blood is restricted from clotting by the presence of anti-coagulants, then the fluid is plasma.
After blood is collected from a patient it is divided into a number of fragments. By dividing blood into proteins like albumin, white blood cells and red blood cells clinics are assisted in curing patients. An example is a patient with liver failure being provided with blood plasma that contains the clotting factor.
Whilst plasma has an anti-coagulant that prevents clotting, serum does not contain the plasma proteins which play a part in clotting such a fibrinogen which is converted to fibrin. Plasma itself is a yellow fluid which is found in lymph or the intramuscular fluids containing fibrin and various other clotting factors. Separating the various substances of blood is a complicated process. It consists of the plasma being made by spinning a test tube (containing the blood) in centrifuges which will eventually lead to blood cells being insulated at the end of the test tube and the plasma can then be drained off. The plasma has a density of 1.025kg/1 and can be stored for up to 10 years.
Electrophoresis is the process by which the proteins are collected from