Biology Lab
TEXT BOOK OF
BIOCHEMISTRY
For 2nd year Medical Laboratory Technician Course
(INTERMEDIATE VOCATIONAL)
Author :
S. Srinivasa Rao, B.Pharmacy,
Jr. Lecturer in M.L.T., Alluri Sri Rama Raju Govt. Junior College, Shanthi Nagar, Khammam.
Editor :
S. Kamalakar Rao, M. Pharmacy
Senior Lecturer in Pharmacy, Department of Biotechnology, Govt. institute for post diploma courses in engineering and technology, Ramanthapur,Hyderabad.
INDEX
Chapter I. Page No. Instrumental methods of Biochemical analysis ..................... 1 1. Flame Photometry ................................................................... 1 2. Fluorimetry ........................................................................... 10 3. Nephelometry ....................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays ........................................................................ 70 1. Radio immunoassays ............................................................. 70 2. Enzyme linked immunoassays ................................................ 89 IV. Metabolism ............................................................................. 94 1. Carbohydrate metabolism...................................................... 95 2. Lipid metabolism ................................................................. 101 3. Protein metabolism .............................................................. 104 V. Titrimetric methods of quantitative determination, Preparation of varioussolutions used in Titrimetric analysis .............. 109 VI. Liver function tests
BIOCHEMISTRY
For 2nd year Medical Laboratory Technician Course
(INTERMEDIATE VOCATIONAL)
Author :
S. Srinivasa Rao, B.Pharmacy,
Jr. Lecturer in M.L.T., Alluri Sri Rama Raju Govt. Junior College, Shanthi Nagar, Khammam.
Editor :
S. Kamalakar Rao, M. Pharmacy
Senior Lecturer in Pharmacy, Department of Biotechnology, Govt. institute for post diploma courses in engineering and technology, Ramanthapur,Hyderabad.
INDEX
Chapter I. Page No. Instrumental methods of Biochemical analysis ..................... 1 1. Flame Photometry ................................................................... 1 2. Fluorimetry ........................................................................... 10 3. Nephelometry ....................................................................... 12 4. Basic principles and applications of Potentiometry .................. 16 II. Separation techniques ............................................................ 25 1. Chromatography ................................................................... 25 2. Electrophoresis ..................................................................... 53 III. Immunoassays ........................................................................ 70 1. Radio immunoassays ............................................................. 70 2. Enzyme linked immunoassays ................................................ 89 IV. Metabolism ............................................................................. 94 1. Carbohydrate metabolism...................................................... 95 2. Lipid metabolism ................................................................. 101 3. Protein metabolism .............................................................. 104 V. Titrimetric methods of quantitative determination, Preparation of varioussolutions used in Titrimetric analysis .............. 109 VI. Liver function tests
References: 1) Practical Clinical Biochemistry by Harold Varley 2) Medical Laboratory Technology by Praful Godkar 3) Medical Laboratory Technology by Ramnik Sood 4) Medical Laboratory Technology manual by K.M. Samuel 5) Chemistry of natural products by O.P. Agarwal 6) Theory and Practice of industrial Pharmacy by Leon Lachman 7) Human Physiology by Chaterjee 8) Author’s learnings from Dr. C.K.Kokate, Dr. M.C.P ., Dr. A.V.N.Appa Rao, Dr .V.Malla Reddy, Dr .Raghu Rama Rao & Late Dr .Ravi Shankar.