Biology Lab Report

Iodine is a test for starch while Benedict's solution is a test for reducing sugars. When solution A is tested by benidicts test, the clear blue solution changed to a little reddish and brick red precipitate is formed.this result show that solution A is a reducing sugar. When carried out iodine test with solution A, the colourless solution remain unchanged . this tell us that starch is absent is solution A. When solution B is tested with Benedicts test, the clear blue solution remain unchanged, we can conclude that it is a non reducing sugar. When tested with iodine test, the colourless solution changed to dark blue colour.this show that starch is present in solution B. From the above test, we can conclude that solution B is a starch solution so we can use it to carry out the next test. solution B , saliva and hydrochloric acid was used in the second test. The enzyme involed in this test is salivary amylase which can found in saliva. Salivary Amylase, is called ptyalin it’s to break down starch to sugar. Things we ate is broken down in mouth by amylase. Amylase hydrolysed starch into a reducing sugar which can give beneduct test a positive result. Body temperature is the optimal temperature for the action of amylase. Enzymes are catalysts for any reactions. They provide an alternative way of lower activation energy to speed up the reaction. When the temperature is low, the kinetic energy of substrate and enzyme is low, this take long time for both of the substance to bind together so the rate of reaction is low. When the temperature is high, the kinetic energy of the substance and enzyme is high too. The substrate and enzyme collide more often with each other. The rate of the both substance to bind is increase too. When the temperature reach optimal, the kinetic energy and collision increase but the rate of the substrate-enzyme bind to form is decrease because the amino acid in the molecule vibrate strongly. When the temperature is too high and exceed the