Lab Report Biology 1 And Lab
Enzymes
Introduction
This study allows the investigation of enzymatic reactions behavior.
An enzyme is a protein catalyst reaction by lowering the activation energy required for that reaction. The enzyme is unaltered at the completion of the reaction. In this stimulation the amount of product produced during the course of an enzymatic product produced during the course of an enzymatic reaction will be measured.
Hypothesis 1:
What is the estimate optimal of ph?
Hypothesis 2:
What is the estimate optimal of temperature?
Hypothesis 3:
How does the concentration of enzyme influence the rate of reaction?
Why do you think this occurs?
Materials
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Substrate solution
Cuvette
Spectrophotometer
Timer
Enzyme solutions
Pipette for transferring fluids
Thermometer
Methods
● There is a choice of six different enzyme & substrate combinations in which the temperature will vary at which the reaction as well as the enzyme & substrate concentrations.
pH:
1. A SUbstrate B/ Enzyme B was selected along with setting the wavelength to substrate vial/ 680 & the spectrophotometer to read light absorption.
2. The temperature was set to 25 degrees & set to different experimental pH values starting at value 2 through value 10 (all even #’s)
3. Transferred 25 ml of the substrate B into the cuvette using the pipette.
4. Referring to the timer to determine when 1 minute has passed transferred 5 ml of enzyme B into the cuvette and quickly into the sample holder of the spectrophotometer.
5. Recorded the absorption value at the 1 minute mark
6. Started a new experiment by removing the cuvette from the spectrophotometer and clicking the “New Experiment” button to repeat and continue the steps for the remaining pH values.
7. Determined the estimate of optimal pH after the entire experiment for as a result.
Temperature:
1. Used the same SUbstrate/ Enzyme B & set the pH value to the optimal pH value - 4 from the pH
Introduction
This study allows the investigation of enzymatic reactions behavior.
An enzyme is a protein catalyst reaction by lowering the activation energy required for that reaction. The enzyme is unaltered at the completion of the reaction. In this stimulation the amount of product produced during the course of an enzymatic product produced during the course of an enzymatic reaction will be measured.
Hypothesis 1:
What is the estimate optimal of ph?
Hypothesis 2:
What is the estimate optimal of temperature?
Hypothesis 3:
How does the concentration of enzyme influence the rate of reaction?
Why do you think this occurs?
Materials
●
●
●
●
●
●
●
Substrate solution
Cuvette
Spectrophotometer
Timer
Enzyme solutions
Pipette for transferring fluids
Thermometer
Methods
● There is a choice of six different enzyme & substrate combinations in which the temperature will vary at which the reaction as well as the enzyme & substrate concentrations.
pH:
1. A SUbstrate B/ Enzyme B was selected along with setting the wavelength to substrate vial/ 680 & the spectrophotometer to read light absorption.
2. The temperature was set to 25 degrees & set to different experimental pH values starting at value 2 through value 10 (all even #’s)
3. Transferred 25 ml of the substrate B into the cuvette using the pipette.
4. Referring to the timer to determine when 1 minute has passed transferred 5 ml of enzyme B into the cuvette and quickly into the sample holder of the spectrophotometer.
5. Recorded the absorption value at the 1 minute mark
6. Started a new experiment by removing the cuvette from the spectrophotometer and clicking the “New Experiment” button to repeat and continue the steps for the remaining pH values.
7. Determined the estimate of optimal pH after the entire experiment for as a result.
Temperature:
1. Used the same SUbstrate/ Enzyme B & set the pH value to the optimal pH value - 4 from the pH