biooo
Exercise No. 1
Micrometry: The Science of Standardized and Calibrated Micrometric Measurements
(The Jellybeans) Joaquim B. Aganon, Glaiza S. Ibanez
(14 August 2014)
(21 August 2014)
I. Introduction
Microscopes and the field of Science are two inseparable things. Almost all Sciences are associated with the use of a microscope, a device use to view objects which are not visible to the naked eye. The term microscope came from the Greek term “Mikros” which means “small” and “Skopien” which means “to examine” (Vocabulary.com, 2014). Today, microscopes are of great importance especially on the field of Biological Science. One of its major use is to determine the sizes of microorganisms which are of great importance in understanding certain biological phenomena.
Getting the actual size of microscopic objects such as microorganisms using calibrated eyepiece micrometer is termed micrometry (Ochei, et. Al., 2000). It is important for investigators across a number of disciplines. A biologist, for example, needs to know the exact size of two organisms to provide an intelligent comparison (i.e. Allometry). The intent of this activity is to create an awareness in proper handling techniques and correct use of a microscope to students pursuing courses in the field of Biological Sciences. This activity is also intended to teach students microscope calibration techniques.
II. Materials and Methods To able to obtain the exact measurement of a specimen, the ocular micrometer should be calibrated first. By doing so, the calibration constant (CC) is obtained. To get the calibration constant, a stage micrometer, an ocular micrometer and a microscope is used. The ocular micrometer was placed inside the ocular lens of the microscope and the stage micrometer on the stage. The field was adjusted so that a line in the ocular micrometer is superimposing a line in the stage micrometer. Once this was set, the number of divisions in both micrometers from the first superimposing lines
Micrometry: The Science of Standardized and Calibrated Micrometric Measurements
(The Jellybeans) Joaquim B. Aganon, Glaiza S. Ibanez
(14 August 2014)
(21 August 2014)
I. Introduction
Microscopes and the field of Science are two inseparable things. Almost all Sciences are associated with the use of a microscope, a device use to view objects which are not visible to the naked eye. The term microscope came from the Greek term “Mikros” which means “small” and “Skopien” which means “to examine” (Vocabulary.com, 2014). Today, microscopes are of great importance especially on the field of Biological Science. One of its major use is to determine the sizes of microorganisms which are of great importance in understanding certain biological phenomena.
Getting the actual size of microscopic objects such as microorganisms using calibrated eyepiece micrometer is termed micrometry (Ochei, et. Al., 2000). It is important for investigators across a number of disciplines. A biologist, for example, needs to know the exact size of two organisms to provide an intelligent comparison (i.e. Allometry). The intent of this activity is to create an awareness in proper handling techniques and correct use of a microscope to students pursuing courses in the field of Biological Sciences. This activity is also intended to teach students microscope calibration techniques.
II. Materials and Methods To able to obtain the exact measurement of a specimen, the ocular micrometer should be calibrated first. By doing so, the calibration constant (CC) is obtained. To get the calibration constant, a stage micrometer, an ocular micrometer and a microscope is used. The ocular micrometer was placed inside the ocular lens of the microscope and the stage micrometer on the stage. The field was adjusted so that a line in the ocular micrometer is superimposing a line in the stage micrometer. Once this was set, the number of divisions in both micrometers from the first superimposing lines
References: Ochei, et. al., 2000. Medical Laboratory Science : Theory And Practice. New Delhi: Tata McGraw-Hill Publishing Company Limited Rottenfusser, E. and Wilson, et. al., 2014. Education in Microscopy and Digital Imaging. Retrieved 20 August 2014 from,http://zeiss-campus.magnet.fsu.edu/articles/basics/resolution.htmlﾧ Vocabulary.com, 2014. Microscopic. Retrieved, 20 Augusr, 2014 from , http://www.vocabulary.com/dictionary/microscopic Microscope World, 2014. Parfocal Microscope Objectives. Retrieved, 21 August 2014 from, https://www.microscopeworld.com/t-parfocal_microscope_objectives.aspxﾧ