Lab 1 Microscope
Microbiology Kit (MBK) – Lab Report Observing Bacteria and Blood
Questions: List the following parts of the microscope and describe the function of each
A. Ocular Lens- the lens on the top of the microscope that look into with 10x or 15x power. B. Body Tube- Connects eye piece to the objective lens C. Revolving Nosepiece- holds two or more objectives lenses and can be rotated easily to change power D. Objective lenses- 4x, 10x, 40x, and 100x powers E. Stage- The flat plate where the slides are placed for observation F. Diaphragm- Generally a five hold disc placed under the stage. Used to vary the intensity and size of the cone of light to see the slide. G. Illuminator- A light source, used to reflect light from an external light source up through the bottom of the stage. H. Coarse Focus Knob- Rough focus knob on the microscope used to move the objective lenses towards or away from the specimen. I. Fine Focus Knob- Knob used to fine tune the focus on the specimen, used after the coarse focus knob. J. Arm- Part of microscope that connects the tube to the base, used when carrying. K. Stage Clip- Clips on the stage used to hold the slide into place L. Base- bottom support of the microscope B.
Define the following microscopy terms:
a. Focus: Means of moving the specimen closer or further away from the objective lens and get a sharper image b. Resolution: Ability of a lens system to show details of the object c. Contrast: Darkness of the background relative to the specimen
C. Describe your observations from fresh yogurt slide you prepared in Part III. We did not do the fresh yogurt slide.
D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, describe them. The prepared yogurt slide was purple spheres and commas covering the whole slide.
E. Describe the four main bacterial shapes The four main bacterial shapes were spheres, rods, commas, and spirals.
F. What are the common arrangements
Questions: List the following parts of the microscope and describe the function of each
A. Ocular Lens- the lens on the top of the microscope that look into with 10x or 15x power. B. Body Tube- Connects eye piece to the objective lens C. Revolving Nosepiece- holds two or more objectives lenses and can be rotated easily to change power D. Objective lenses- 4x, 10x, 40x, and 100x powers E. Stage- The flat plate where the slides are placed for observation F. Diaphragm- Generally a five hold disc placed under the stage. Used to vary the intensity and size of the cone of light to see the slide. G. Illuminator- A light source, used to reflect light from an external light source up through the bottom of the stage. H. Coarse Focus Knob- Rough focus knob on the microscope used to move the objective lenses towards or away from the specimen. I. Fine Focus Knob- Knob used to fine tune the focus on the specimen, used after the coarse focus knob. J. Arm- Part of microscope that connects the tube to the base, used when carrying. K. Stage Clip- Clips on the stage used to hold the slide into place L. Base- bottom support of the microscope B.
Define the following microscopy terms:
a. Focus: Means of moving the specimen closer or further away from the objective lens and get a sharper image b. Resolution: Ability of a lens system to show details of the object c. Contrast: Darkness of the background relative to the specimen
C. Describe your observations from fresh yogurt slide you prepared in Part III. We did not do the fresh yogurt slide.
D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, describe them. The prepared yogurt slide was purple spheres and commas covering the whole slide.
E. Describe the four main bacterial shapes The four main bacterial shapes were spheres, rods, commas, and spirals.
F. What are the common arrangements