Compound Light Microscope
INSTRUCTIONAL OBJECTIVES
1. Define
Ocular: (eyepiece)
Objective: provides magnification of 4, 10, 40, and 100 power
Low power: 4X 10X
High-and-dry: 40X
Oil immersion objective: 100X
Fine adjustment: is essential for final, sharp focusing
Coarse adjustment: is used initially for rough focusing
Parfocal: as you cane from one objective to another you will find that your microscope is very close to a final focus
Sub stage condenser: light focusing lens
Iris diaphragm: used to regulate the intensity of light entering the lens system of the microscope
Mechanical stage: allows total movement of the slide by turning bot knobs beneath the left side of the stage, one for to-and-fro, one for the left and right positioning
Resolution (resolving power): is the ability to distinguish two points as separate when they are close together
Wavelength: the distance between similar points along a wave
Compound light microscope: is an optical instrument comprised of more than one lens and uses light to illuminate the object under study
Electron microscope: can provide magnifications in the vicinity of 1 to 2 million power
Cell: is a fundamental anatomic until in which all life is contained
2. List and describe the features of four different types of compound light microscopes.
Bright field Microscope: Field is bright wit lit and the object under study is usually darkened by staining
Dark field Microscope: field is dark and the object under study is lit and sometimes alive
Phase Contrast Microscope: contrasting lit intensities are provided by the optical system, even if the object is unstained
Fluorescent Microscope: uses ultraviolet lit for illumination, as special applications, e.g., fluorescent antibody slides
3. State the upper limit of effective magnification of most compound light microscopes.
The upper limit of magnification of many compound light microscopes is approximately 2000 power, that is, the microscope will magnify the image of an object
1. Define
Ocular: (eyepiece)
Objective: provides magnification of 4, 10, 40, and 100 power
Low power: 4X 10X
High-and-dry: 40X
Oil immersion objective: 100X
Fine adjustment: is essential for final, sharp focusing
Coarse adjustment: is used initially for rough focusing
Parfocal: as you cane from one objective to another you will find that your microscope is very close to a final focus
Sub stage condenser: light focusing lens
Iris diaphragm: used to regulate the intensity of light entering the lens system of the microscope
Mechanical stage: allows total movement of the slide by turning bot knobs beneath the left side of the stage, one for to-and-fro, one for the left and right positioning
Resolution (resolving power): is the ability to distinguish two points as separate when they are close together
Wavelength: the distance between similar points along a wave
Compound light microscope: is an optical instrument comprised of more than one lens and uses light to illuminate the object under study
Electron microscope: can provide magnifications in the vicinity of 1 to 2 million power
Cell: is a fundamental anatomic until in which all life is contained
2. List and describe the features of four different types of compound light microscopes.
Bright field Microscope: Field is bright wit lit and the object under study is usually darkened by staining
Dark field Microscope: field is dark and the object under study is lit and sometimes alive
Phase Contrast Microscope: contrasting lit intensities are provided by the optical system, even if the object is unstained
Fluorescent Microscope: uses ultraviolet lit for illumination, as special applications, e.g., fluorescent antibody slides
3. State the upper limit of effective magnification of most compound light microscopes.
The upper limit of magnification of many compound light microscopes is approximately 2000 power, that is, the microscope will magnify the image of an object