Observing Bacteria and Blood
MBK – Lab Report Name: _Bri White_________ Section: ___________________
Observing Bacteria and Blood
Questions:
A. List the following parts of the microscope and describe the function of each
A- Eyepiece: Viewing and identifying objects within the viewing field
B- Main Tube: Connects eyepiece lenses to objective lenses
C- Nosepiece: Holds objective lens and rotates them
D- Objective Lens: Provides different focal lengths
E- Stage: Holds the specimen or slide
F- Diaphragm: Controls the volume of light reaching the specimen or slide
G- Light Source: illuminates slide work
H- Course Adjustment: For initial focusing of the viewing field
I- Fine Adjustment: For final focusing of the viewing field
J- Arm: Connects the base to the main tube; provides upright stability
K- Stage Knobs or Clips: Adjust the slides position on the stage
L- Base: Provides lateral stability for viewing
B. Define the following microscopy terms: a. a. Focus: to position the objective lens at the proper distance from the specimen b. b. Resolution: the closest two objects can be before they are no longer detected as separate objects (usually measured in nm) c. c. Contrast: related to the illumination system and can be adjusted by changing the intensity of the light and diaphragm aperture; the difference in lighting between adjacent areas of the specimen
C. Describe your observations from fresh yogurt slide you prepared in Part III. While viewing under 4x magnification, you can see the presence of cocci and bacillus bacteria strains. After increasing the magnification to 10x, you can view these shapes more clearly, but it is not until 40x magnification that you can see the cooci exist in pairs and strands, in other words, diplococci and streptococci are present in the yogurt slide. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, describe them. No observable differences.
E.
Observing Bacteria and Blood
Questions:
A. List the following parts of the microscope and describe the function of each
A- Eyepiece: Viewing and identifying objects within the viewing field
B- Main Tube: Connects eyepiece lenses to objective lenses
C- Nosepiece: Holds objective lens and rotates them
D- Objective Lens: Provides different focal lengths
E- Stage: Holds the specimen or slide
F- Diaphragm: Controls the volume of light reaching the specimen or slide
G- Light Source: illuminates slide work
H- Course Adjustment: For initial focusing of the viewing field
I- Fine Adjustment: For final focusing of the viewing field
J- Arm: Connects the base to the main tube; provides upright stability
K- Stage Knobs or Clips: Adjust the slides position on the stage
L- Base: Provides lateral stability for viewing
B. Define the following microscopy terms: a. a. Focus: to position the objective lens at the proper distance from the specimen b. b. Resolution: the closest two objects can be before they are no longer detected as separate objects (usually measured in nm) c. c. Contrast: related to the illumination system and can be adjusted by changing the intensity of the light and diaphragm aperture; the difference in lighting between adjacent areas of the specimen
C. Describe your observations from fresh yogurt slide you prepared in Part III. While viewing under 4x magnification, you can see the presence of cocci and bacillus bacteria strains. After increasing the magnification to 10x, you can view these shapes more clearly, but it is not until 40x magnification that you can see the cooci exist in pairs and strands, in other words, diplococci and streptococci are present in the yogurt slide. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, describe them. No observable differences.
E.