Microbiology Labs
MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________
EXPERIMENT 1
TITLE: Observing Bacteria and Blood
OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood.
PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood:
After reviewing the section of the manual as instructed I cleaned the ocular lenses and prepared the slides. I made required adjustments to the microscope and ocular distances for view during experiment. I practiced using six prepared slides that were in the kit to ensure I was viewing the slides accordingly. I placed a drop of oil onto the slide and rotated it until bringing it to fine focus on the microscope. I then observed the same slides in part 1 but I added the oil immersion to view the differences. The difference was in the magnification and the ability to see more of the substances in finer view. I then made my own sample of yogurt, I left it undisturbed for 24 hours and observed it under the microscope. The sample was not was the right consistency. I viewed the specimen under the microscope. I pricked my finger with the sterilized pin and placed my blood on a slide with a slip cover. I observed the specimen under the microscope. I placed the prepared slide with my blood on the microscope stage, and brought it into focus on low power. I saw a lot of tiny particles that appeared to be blood cells through the microscope.
QUESTIONS:
Compare your observations from the fresh yogurt slide and the prepared yogurt slide. Are there differences? Why or why not? The fresh yogurt appeared to have less growth than the prepared slide of yogurt. This is because the prepared yogurt was no longer gestating and showed an increase in bacterial numbers.
Were you able to identify specific bacterial morphologies? Which
EXPERIMENT 1
TITLE: Observing Bacteria and Blood
OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood.
PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood:
After reviewing the section of the manual as instructed I cleaned the ocular lenses and prepared the slides. I made required adjustments to the microscope and ocular distances for view during experiment. I practiced using six prepared slides that were in the kit to ensure I was viewing the slides accordingly. I placed a drop of oil onto the slide and rotated it until bringing it to fine focus on the microscope. I then observed the same slides in part 1 but I added the oil immersion to view the differences. The difference was in the magnification and the ability to see more of the substances in finer view. I then made my own sample of yogurt, I left it undisturbed for 24 hours and observed it under the microscope. The sample was not was the right consistency. I viewed the specimen under the microscope. I pricked my finger with the sterilized pin and placed my blood on a slide with a slip cover. I observed the specimen under the microscope. I placed the prepared slide with my blood on the microscope stage, and brought it into focus on low power. I saw a lot of tiny particles that appeared to be blood cells through the microscope.
QUESTIONS:
Compare your observations from the fresh yogurt slide and the prepared yogurt slide. Are there differences? Why or why not? The fresh yogurt appeared to have less growth than the prepared slide of yogurt. This is because the prepared yogurt was no longer gestating and showed an increase in bacterial numbers.
Were you able to identify specific bacterial morphologies? Which