Buhinia Racemosa Lab Report

ABSTRACT Nature has been a source of medicinal agents for thousands of years and an impressive number of modern drugs have been isolated from natural sources Plants used for traditional medicine contain a wide range of substances that can be used to treat chronic as well as infectious diseases. Clinical microbiologists have great interest in screening of plants for antimicrobial activities and phytochemicals as potential new therapeutics. The use of plant extract for medical treatments is enjoying great popularity since 1990s when people realized that the effective life span of antibiotic is limited and over prescription and misuse of traditional antibiotics are causing microbial resistance. The antimicrobial activities of plant extracts
After 24 hours of the colonies were picked up and preliminary tests like gram staining, motility, catalase and oxidase tests were performed. Further these isolates were identified as per standard biochemical methods.

COLLECTION OF MEDICINAL PLANTS
The whole plant of Bauhinia racemosa (Mandharai) was collected and was processed for its evaluation of antimicrobial activity against isolated organisms.
Processing Of The Plant:
The whole plants along with its leaves stems, root parts were selected and they were shade dried in a clean environment for few days. The dried plant parts was collected and was powdered and was stored in a clean container for further screening and extraction procedures.

PLANT EXTRACTION METHODS Three solvents were used for the preparation of the extracts - distilled water, acetone, ethanol at four different concentrations. Plant powder was weighed and mixed with the three solvents to attain four different concentrations viz., 20%, 40% 60% and 80% respectively. These extracts were further used for the
S.aureus gave a maximum zone size of 15mm in diameter at 80% concentration of plant extract with acetone. CoNS gave a zone size ranging from 3-9mm with different concentrations of plant extract with ethanol, 2-11mm with acetone and 1-6mm with plant extract made with distilled water. CoNS gave a maximum zone size of 11mm in diameter at 80% concentration of plant extract with acetone. P.aeruginosa gave a zone size ranging from 9-20mm with different concentrations of plant extract with ethanol, 8-16mm with acetone and 1-5mm with plant extract made with distilled water. P.aeruginosa gave a maximum zone size of 20mm in diameter at 80% concentration of plant extract with ethanol. Klebsiella spp gave a zone size ranging from 4-9mm with different concentrations of plant extract with ethanol, 3-10mm with acetone and 2-8mm with plant extract made with distilled water. Klebsiella spp gave a maximum zone size of 10mm in diameter at 80% concentration of plant extract with acetone. (Table